抗磷酸化SHIP抗体,多克隆

兔多克隆抗体,抗人、小鼠磷酸化SHIP

产品号 #(选择产品)

产品号 #60142_C

兔多克隆抗体,抗人、小鼠磷酸化SHIP

总览

Phospho-SHIP是SHIP的高度磷酸化形式(也称为SHIP1)。含SH2结构域的肌醇磷酸酶(SHIP)是一种分子量为145 kDa的造血系统限制性蛋白,在细胞因子、生长因子、趋化因子及免疫受体刺激后被酪氨酸磷酸化,并与适配蛋白Shc结合。SHIP通过水解磷脂酰肌醇-3激酶(PI3K)产生的关键第二信使PI-3,4,5-P3(PIP3)生成PI-3,4-P2,从而作为PI3K信号通路的重要负调控因子。该抗体可识别小鼠和人源的酪氨酸磷酸化SHIP蛋白。

亚型
一抗
 
靶抗原
PhosphoSHIP
 
别名
p150Ship;磷脂酰肌醇3,4,5-三磷酸5-磷酸酶1;SHIP-1;SHIP1;SIP-145; s-SHIP
 
活性物种
人,小鼠
 
偶联
未偶联的
 
宿主物种

 
细胞类型
粒细胞及其亚群,淋巴细胞,髓系细胞
 
种属
人,小鼠
 
应用
流式细胞术,免疫印迹,免疫荧光,免疫沉淀
 
研究领域
免疫
 
克隆
Not applicable
 
基因编号
3635 (human),16331 (mouse)
 
同种型
Not applicable
 

Data Figures

(A) Flow cytometry analysis of bone marrow derived mast cells (BMMCs) from normal (+/+) or SHIP knockout (-/-) mice. Cells were stimulated with stem cell factor (SCF; filled histogram) or left unstimulated (solid line historgram), and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(B) BMMCs were either stimulated with SCF or left unstimulated, and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(C) Western blot analysis of total cell lysates from human TF-1 cells stimulated with IL-3, mouse WEHI-231 B cells stimulated with anti-IgM, and mouse BMMCs stimulated with SCF, and probed with Anti-Phospho-SHIP Antibody, Polyclonal. The blot was re-probed with an anti-SHIP antibody to show equal loading of unstimulated (C) and stimulated (S) samples. Phospho-SHIP has a predicted molecular mass of 145 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60142
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60142
Lot #
All
Language
English

Resources and Publications

Educational Materials (2)

更多信息

更多信息
Species Human, Mouse
Clone Not applicable
Gene Id 3635 (human), 16331 (mouse)
Alternative Names p150Ship; phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1; SHIP-1; SHIP1; SIP-145; s-SHIP
Isotype Not applicable
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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