产品号 #60150_C
Rat monoclonal IgG2a antibody against mouse Ly-49A
概述
The YE1/32.8.5 antibody reacts with Ly-49A, a member of the C-type lectin family of proteins. Ly-49A is a type II transmembrane protein expressed as a 44 kDa disulfide-linked homodimer on the surface of subsets of natural killer (NK) cells, NK-T cells, and T cells by some mammalian species, but not humans. It functions to inhibit NK cell activation by binding to certain haplotypes of MHC class I molecules on target cells, specifically H-2Dd and H2Dk, which results in phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain and the recruitment of tyrosine phosphatases, such as SHP-1 and SHP-2. While many anti-Ly-49A antibodies are strain-specific, the YE1/32.8.5 antibody reacts with NK cells from all strains of mice tested. This antibody inhibits the binding of Ly-49A to its ligands and reverses the inhibition of NK cytotoxicity mediated by specific MHC class I molecules on target cells. It very weakly cross-reacts with Ly-49D, but the levels of cross-reactivity are deemed too low to affect flow cytometric analysis of NK cells for functional studies of Ly-49A.
Subtype
Primary Antibodies
Target Antigen
Ly-49A
Alternative Names
Killer cell lectin-like receptor subfamily A member 1, Klra1, Lymphocyte antigen 49a, Natural killer cell receptor Ly49A, T lymphocyte antigen A1
Reactive Species
Mouse
Conjugation
Unconjugated
Host Species
Rat
Cell Type
NK Cells
Species
Mouse
Application
Flow Cytometry, Immunoprecipitation
Area of Interest
Immunology
Clone
YE1/32.8.5
Gene ID
16627
Isotype
IgG2a, kappa
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-49A Antibody, Clone YE1/32.8.5 Catalog #
60150 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-49A Antibody, Clone YE1/32.8.5 Catalog #
60150 Lot #
All Language
English Resources and Publications
Educational Materials (2)
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