产品号 #60020_C
Rat monoclonal IgM antibody against mouse CD49b (integrin α2)
概述
The DX5 antibody reacts with murine CD49b (integrin α2), an ~150 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD29 (integrin β1) to form the heterodimeric CD49b/CD29 complex known as VLA-2, a receptor for extracellular matrix proteins such as collagen, E-cadherin, fibronectin, and laminin. CD49b is highly expressed by platelets and is found on a majority of NK cells, on NKT cells, and on a small subset of CD8+ T cells; the latter population increases substantially following viral infection. CD49b is also expressed by several tissues, including intestine, kidney, mammary gland, and lung. The DX5 antibody is particularly useful for identifying NK cells in mice lacking the NK1.1 antigen. Binding of the DX5 antibody has not been observed to affect the function of the VLA-2 integrin. DX5 binding is, however, blocked by the clone HMα2 antibody.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755).
Subtype
Primary Antibodies
Target Antigen
CD49b (Integrin α2)
Alternative Names
α2 integrin, Integrin α2 chain, VLA-2α chain
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, Pacific Blue, PE, Unconjugated
Host Species
Rat
Cell Type
NK Cells
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Immunohistochemistry
Area of Interest
Immunology
Clone
DX5
Gene ID
16398
Isotype
IgM, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488 (Catalog #60074AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.
Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE (Catalog #60074PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.
Figure 3. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118 (Catalog #60074), followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 antibody, Clone 30-F11, PE.
Figure 4. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC (Catalog #60074AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.
Figure 5. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin (Catalog #60074BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.
Figure 6. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC (Catalog #60074FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 7. Data for PB-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgM, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, APC Catalog #
60020AZ, 60020AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, Biotin Catalog #
60020BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, PE Catalog #
60020PE, 60020PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5 Catalog #
60020 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, APC Catalog #
60020AZ, 60020AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, Biotin Catalog #
60020BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5, PE Catalog #
60020PE, 60020PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD49b Antibody, Clone DX5 Catalog #
60020 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Total Lymphocyte Research
Resources and Publications
Educational Materials (3)
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Legal Statement: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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