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抗小鼠IFN-γ抗体，克隆XMG1.2，APC

抗小鼠IFN-gamma的大鼠单克隆IgG1抗体
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产品号 #100-0277_C

抗小鼠IFN-gamma的大鼠单克隆IgG1抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

XMG1.2抗体与小鼠干扰素γ (IFN-γ)发生反应，IFN-γ是一种糖基化的II型干扰素，其活性形式包含约20 kDa亚基的同型二聚体。IFN‑γ 主要由活化的 T 淋巴细胞和自然杀伤（NK）细胞在抗原刺激下产生，这些抗原可包括同种异体抗原、有丝分裂原及肠毒素等。IFN-γ与IFN-γ受体的α亚基具有高亲和力结合，导致配体诱导的四聚体α2β2受体复合物的组装，该复合物能够通过激活JAK-STAT途径介导细胞对细胞因子的反应。IFN-γ受体在所有有核细胞表面表达。IFN-γ在多种生物过程中发挥重要功能，包括巨噬细胞活化，介导对病毒和细菌的免疫反应，以及调节细胞增殖和凋亡。当与适当的二抗配对时，XMG1.2抗体适用于流式细胞术等方法检测细胞内IFN-γ。XMG1.2抗体也被广泛用于各种检测中，以中和IFN-γ的功能活性。

亚型
一抗
 
靶抗原
IFNγ
 
别名
IFN-γ，免疫干扰素，干扰素-γ，巨噬细胞活化因子（MAF），T细胞干扰素，II型干扰素
 
活性物种
小鼠
 
偶联
APC，Biotin 或 生物素，FITC，PE
 
宿主物种
大鼠
 
细胞类型
树突状细胞（DCs），巨噬细胞，NK 细胞，T 细胞
 
应用
ELISAs，ELISpot，流式细胞术
 
研究领域
免疫
 
克隆
XMG1.2
 
基因编号
15978
 
同种型
IgG1，kappa
 

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Data Figures

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure 1. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, Biotin.

Figure 2. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-γ Antibody, Clone XMG1.2, Biotin, followed by streptavidin (SAV) PE.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody, Clone XMG1.2, FITC.

Figure 3. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC

Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC.

Figure showing flow cytometry analysis of anti-mouse IFN-gamma antibody Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

Figure 4. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE

Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0278, 100-0277
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0278, 100-0277
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research

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更多信息
Clone XMG1.2
Gene Id 15978
Alternative Names IFN-γ, Immune interferon, Interferon-gamma, Macrophage-activating factor (MAF), T cell interferon, Type II interferon
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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