产品号 #60045_C
小鼠抗人、恒河猴、食蟹猴CD90（Thy-1）单克隆IgG1抗体
总览
5E10抗体与CD90 （Thy-1）反应，CD90 是一种 GPI 锚定的膜糖蛋白，在两个位点进行 N-糖基化，生成分子量在 25–37 kDa 范围内的蛋白。CD90在信号转导、细胞粘附和迁移、神经突生长、T细胞活化、肿瘤抑制、造血干细胞增殖和分化抑制等方面发挥作用。它是已知的β2和β3整合素的配体，并上调纤维连接蛋白、骨连接蛋白和血小坂反应蛋白的合成。CD90广泛表达，存在于人胸腺细胞、神经元、部分神经胶质细胞、成纤维细胞、活化内皮细胞、某些白血病细胞系以及人外周血中一小部分（<1%）CD3+CD4+ T 细胞中。CD90还在胎肝、脐带血、骨髓和动员的外周血细胞中小部分 CD34+ 细胞中表达。CD90被认为是造血干细胞和祖细胞的重要标志物，与CD34等其他标志物结合，可用于流式细胞术（FACS）鉴定和分选这些细胞。
该抗体克隆已被验证用于标记使用MesenCult™增殖试剂盒(产品号：#05411)和MesenCult™-XF培养基（产品号：#05420）培养的人间充质细胞。
亚型
一抗
靶抗原
CD90 (Thy-1)
别名
CDw90，Thy-1，Thy1
活性物种
Baboon，Cynomolgus，Dog，人，Macaque，Pig，Rhesus
偶联
APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
宿主物种
小鼠
细胞类型
造血干/祖细胞，乳腺细胞，间充质干/祖细胞
种属
人，非人灵长类，其它细胞系
应用
CyTOF，ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
研究领域
上皮细胞研究，干细胞生物学
克隆
5E10
基因编号
7070
同种型
IgG1，kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell 2018 JAN
Abstract
Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previously classified as interferon (IFN) stimulated genes (ISGs) but that expression is intrinsic, as stem cells are refractory to interferon. This intrinsic ISG expression varies in a cell-type-specific manner, and many ISGs decrease upon differentiation, at which time cells become IFN responsive, allowing induction of a broad spectrum of ISGs by IFN signaling. Importantly, we show that intrinsically expressed ISGs protect stem cells against viral infection. We demonstrate the in vivo importance of intrinsic ISG expression for protecting stem cells and their differentiation potential during viral infection. These findings have intriguing implications for understanding stem cell biology and the evolution of pathogen resistance.
更多信息
| Species | Human, Non-Human Primate, Other |
|---|---|
| Clone | 5E10 |
| Gene Id | 7070 |
| Alternative Names | CDw90, Thy-1, Thy1 |
| Isotype | IgG1, kappa |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
