抗人CD51抗体,克隆号NKI-M9

小鼠抗人CD51(整合素αV)单克隆IgG2a抗体

产品号 #(选择产品)

产品号 #60043_C

小鼠抗人CD51(整合素αV)单克隆IgG2a抗体

总览

NKI-M9抗体与CD51 (玻连蛋白受体 α 链或整合素 αV)反应。CD51是一种I型跨膜糖蛋白,含有两个亚基(125 kDa和24 kDa),这两个亚基是由翻译后加工产生的,并通过二硫键连接。CD51与整合素β1 (CD29)、β3 (CD61)、β5、β6或β8非共价结合,形成可与细胞外基质成分结合的异源二聚体细胞黏附受体,这些成分包括纤维蛋白原、胶原蛋白、纤维连接蛋白、层粘连蛋白、骨桥蛋白、血小板反应蛋白、玻连蛋白、血管性血友病因子。例如,CD51和CD61结合形成整合素αv/β3受体,主要介导对玻连蛋白的结合。除了介导粘附和细胞骨架重构功能外,含有cd51的整合素还在信号转导中发挥作用,从而调控细胞增殖、分化和迁移等过程。整合素介导的反应需要配体结合和配体诱导的受体聚集共同参与。CD51 广泛表达于多种细胞类型中,包括内皮细胞、成纤维细胞、单核细胞、巨噬细胞、血小板(表达水平相对较低)和破骨细胞。此外,在肝癌、黑色素瘤和神经母细胞瘤细胞中也可检测到 CD51 的表达。

亚型
一抗
 
靶抗原
CD51 (Integrin αV)
 
别名
αV 整合素,整合素 αV,纤维连接蛋白受体 α 链
 
活性物种

 
偶联
Biotin 或 生物素,FITC,PE,未偶联的
 
宿主物种
小鼠
 
细胞类型
间充质干/祖细胞
 
种属

 
应用
ELISAs,流式细胞术,功能学筛选,免疫沉淀
 
研究领域
干细胞生物学
 
克隆
NKI-M9
 
基因编号
3685
 
同种型
IgG2a,kappa
 

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of HT1080 human fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC­173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

Flow cytometry analysis of human HT-1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, FITC (filled historgram) or a mouse IgG2a, kappa FITC isotype control antibody (open histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, PE (filled histogram) or a mouse IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60043FI, 60043FI.1
Lot #
All
Language
English
Catalog #
60043PE, 60043PE.1
Lot #
All
Language
English
Catalog #
60043
Lot #
All
Language
English
Catalog #
60043BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043FI, 60043FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043PE, 60043PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043BT
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

更多信息

更多信息
Species Human
Clone NKI-M9
Gene Id 3685
Alternative Names αV integrin, integrin αV, vitronectin receptor α chain
Isotype IgG2a, kappa
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