产品号 #60043_C
小鼠抗人CD51(整合素αV)单克隆IgG2a抗体
NKI-M9抗体与CD51 (玻连蛋白受体 α 链或整合素 αV)反应。CD51是一种I型跨膜糖蛋白,含有两个亚基(125 kDa和24 kDa),这两个亚基是由翻译后加工产生的,并通过二硫键连接。CD51与整合素β1 (CD29)、β3 (CD61)、β5、β6或β8非共价结合,形成可与细胞外基质成分结合的异源二聚体细胞黏附受体,这些成分包括纤维蛋白原、胶原蛋白、纤维连接蛋白、层粘连蛋白、骨桥蛋白、血小板反应蛋白、玻连蛋白、血管性血友病因子。例如,CD51和CD61结合形成整合素αv/β3受体,主要介导对玻连蛋白的结合。除了介导粘附和细胞骨架重构功能外,含有cd51的整合素还在信号转导中发挥作用,从而调控细胞增殖、分化和迁移等过程。整合素介导的反应需要配体结合和配体诱导的受体聚集共同参与。CD51 广泛表达于多种细胞类型中,包括内皮细胞、成纤维细胞、单核细胞、巨噬细胞、血小板(表达水平相对较低)和破骨细胞。此外,在肝癌、黑色素瘤和神经母细胞瘤细胞中也可检测到 CD51 的表达。
亚型
一抗
靶抗原
CD51 (Integrin αV)
别名
αV 整合素,整合素 αV,纤维连接蛋白受体 α 链
活性物种
人
偶联
Biotin 或 生物素,FITC,PE,未偶联的
宿主物种
小鼠
细胞类型
间充质干/祖细胞
种属
人
应用
ELISAs,流式细胞术,功能学筛选,免疫沉淀
研究领域
干细胞生物学
克隆
NKI-M9
基因编号
3685
同种型
IgG2a,kappa
Figure 1. Data for Unconjugated
Flow cytometry analysis of HT1080 human fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for FITC-Conjugated
Flow cytometry analysis of human HT-1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, FITC (filled historgram) or a mouse IgG2a, kappa FITC isotype control antibody (open histogram).
Figure 3. Data for PE-Conjugated
Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, PE (filled histogram) or a mouse IgG2a, kappa PE isotype control antibody (solid line histogram).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Species | Human |
---|---|
Clone | NKI-M9 |
Gene Id | 3685 |
Alternative Names | αV integrin, integrin αV, vitronectin receptor α chain |
Isotype | IgG2a, kappa |
抗人、小鼠CD105(内皮糖蛋白)的小鼠单克隆IgG1抗体
小鼠抗人、恒河猴、食蟹猴CD90(Thy-1)单克隆IgG1抗体
小鼠单克隆IgG2a, kappa同型对照抗体
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。