产品号 #60044_C
Mouse monoclonal IgG1 antibody against human, rhesus, chimpanzee CD73
概述
The AD2 antibody reacts with human CD73, a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein and ecto-5’-nucleotidase expressed on the surface of subsets of B and T cells, follicular dendritic cells, mesenchymal stem cells, endothelial cells, and epithelial cells. CD73 catalyzes the hydrolysis of 5’-adenosine monophosphate (AMP) to form the bioactive nucleoside adenosine, and plays a pivotal role in the activation of P1 adenosine receptors by regulating extracellular adenosine concentrations. CD73 also appears to function as a co-signaling molecule on T cells and as an adhesion molecule mediating lymphocyte interactions with the endothelium and follicular dendritic cells. CD73 is used as a marker for lymphocyte differentiation, its expression increasing during development. It is also a useful marker for identifying undifferentiated mesenchymal stem cells. CD73 is highly expressed in many types of human and mouse cancers, and has been implicated in the control of tumor growth.
Subtype
Primary Antibodies
Target Antigen
CD73
Alternative Names
5'-nucleotidase, ecto (CD73), Ecto-5'-nucleotidase, L-VAP-2, NT5E
Reactive Species
Chimpanzee, Human, Macaque, Rhesus
Conjugation
APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Area of Interest
Stem Cell Biology
Clone
AD2
Gene ID
4907
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD3 Antibody, Clone UCHT1, PE (Catalog #60011PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, APC and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (Catalog #60011AD). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, APC and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488.
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, Biotin, followed by streptavidin (SAV) APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC (Catalog #60011FI). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC.
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE (Catalog #60011PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE.
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, PE and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (Catalog #60011AD). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, APC Catalog #
60044AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, Biotin Catalog #
60044BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, FITC Catalog #
60044FI, 60044FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, PE Catalog #
60044PE, 60044PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2 Catalog #
60044 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, APC Catalog #
60044AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, Biotin Catalog #
60044BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, FITC Catalog #
60044FI, 60044FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2, PE Catalog #
60044PE, 60044PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD73 Antibody, Clone AD2 Catalog #
60044 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mammary Epithelial Cell Research
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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