产品号 #100-0316_C
抗人、黑猩猩CD45RA的小鼠单克隆IgG2b抗体
HI100抗体可特异性识别CD45的CD45RA异构体上的细胞外表位。CD45是一种I型跨膜糖蛋白,属于蛋白酪氨酸磷酸酶家族(受体1/6亚家族)。编码CD45胞外RA、RB和RC多肽的外显子4、5和6发生可变剪接,产生几种分子量为180 - 240 kDa的同种异构体。切除外显子4可产生分子量约为220 kDa的CD45RA亚型,该亚型在naïve T细胞、B细胞和单核细胞亚群以及骨髓胸腺细胞中表达。CD45RA通过T细胞受体和B细胞受体增强信号传导。HI100抗体通常与抗CD45RO异构体的抗体联合使用,以区分naïve T细胞和记忆T细胞,并可用于识别CD4+淋巴细胞中的抑制/诱导亚群。CD45RA+ T细胞的比例通常随着年龄的增长而下降。
亚型
一抗
靶抗原
CD45RA
别名
B220,CD45,GP180,Lyt-4,Ly-5,LY5,L-CA,LCA,蛋白酪氨酸磷酸酶受体型C,PTPRC,T200
活性物种
Chimpanzee,人
偶联
APC-Cyanine7,Biotin 或 生物素,PE,未偶联的
宿主物种
小鼠
细胞类型
粒细胞及其亚群,造血干/祖细胞,白细胞,淋巴细胞
应用
CyTOF,ELISAs,FACS,流式细胞术,功能学筛选,免疫亲和色谱,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,放射免疫分析,Western印迹
研究领域
免疫
克隆
HI100
基因编号
5788
同种型
IgG2b,kappa
Figure 1. Data for Anti-Human CD45RA Antibody, Clone HI100, APC-Cy7
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 (filled histogram) or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody (solid line histogram).
Figure 2. Data for Anti-Human CD45RA Antibody, Clone HI100, Biotin
Flow cytometry analysis of human PBMCs (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC. (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC. (C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE (filled histogram) or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE (solid line histogram). Viable lymphocytes were gated for analysis.
Figure 3. Data for Anti-Human CD45RA Antibody, Clone HI100, PE
Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE (filled histogram) or a mouse IgG2b, kappa PE isotype control antibody (solid line histogram).
Figure 4. Data for Anti-Human CD45RA Antibody, Clone HI100, Unconjugated
Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC,and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE (solid line histogram).
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Clone | HI100 |
---|---|
Gene Id | 5788 |
Alternative Names | B220, CD45, GP180, Lyt-4, Ly-5, LY5, L-CA, LCA, Protein tyrosine phosphatase receptor type C, PTPRC, T200 |
Isotype | IgG2b, kappa |
小鼠单克隆IgG1抗体,抗人、黑猩猩CD45
小鼠单克隆IgG2b, kappa同型对照抗体
小鼠单克隆IgG2a抗体,抗人、黑猩猩、普通狨猴CD45RO
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