产品号 #19055_C
Immunomagnetic negative isolation of untouched human NK cells
Immunomagnetic negative isolation of untouched human NK cells
Easily and efficiently isolate highly purified human natural killer (NK) cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human NK Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, monocytes, dendritic cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired human NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
For even faster cell isolations, we recommend the EasySep™ Human NK Cell Isolation Kit (Catalog #17955) which isolates cells in just 8 minutes.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood NK Cells, Frozen isolated with EasySep™ Human NK Cell Enrichment Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
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• EasySep™ Magnet (Catalog #18000)
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• “The Big Easy” EasySep™ Magnet (Catalog #18001)
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• Easy 50 EasySep™ Magnet (Catalog #18002)
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• EasyPlate™ EasySep™ Magnet (Catalog 18102)
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• EasyEights™ EasySep™ Magnet (Catalog #18103)
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• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
NK Cells
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.