产品号 #76016_C
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing. ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation. ArciTect™ tracrRNA is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation. It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA. ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes. ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001)
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Genome Editing
Area of Interest
Stem Cell Biology
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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