实验室基础知识
无论您是实验室新手还是需要更新知识，拥有有关基础研究技术的资源可供参考都会有所帮助。 从这里开始了解细胞培养、基本细胞分离方法、基本细胞测定、或其他关键的分子生物学技术。
特色协议
- Tech Tip Sticking Together - Clump Passaging for ES and iPS Cell Maintenance
- Protocol How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture
- Protocol How to Coat Cultureware with Vitronectin XF™ for Pluripotent Stem Cell Culture
- Tech Tip Recommendations for Coating Plates with Matrigel® for Pluripotent Stem Cell Culture
- Tech Tip Using Vitronectin XF™ Defined Matrix for Human Pluripotent Stem Cells
- Protocol How to Thaw Frozen Primary Cells
- Protocol How to Create a Hypoxic Environment for Cell Culture
- Protocol How to Cryopreserve Peripheral Blood Mononuclear Cells (PBMCs)
- Video How to Resuspend Magnetically Labeled Cells After EasySep™ Separation
- Video How to Aspirate an EasyPlate™ Isolation
- Video How to Correctly Pour Liquid from SepMate™ Tubes
- Video How to Prep Flask-Based Samples for EasySep™
- Protocol How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
- Protocol How to Reduce Cell Clumping in Single Cell Suspensions
- Protocol How to Remove Granulocytes from Old Blood Samples
- Protocol How to Generate a Buffy Coat
- Tech TIp Centrifugation Speed and Time for Cells
- Tech TIp Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures
- Tech Tip Reducing Platelet Contamination
- Tech Tip Choosing a Negative or Positive Selection Strategy
- Tech Tip Comparing Cell Separation Platforms from STEMCELL Technologies
- Protocol How to Count Cells with a Hemocytometer
- Protocol How to Count Cell Spheres in Suspension Culture
- Tech Tip Total Nucleated Cell and Viable Cell Counts
- Video Procedure for Setting Up the CFU Assay
- Tech Tip Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage
- Protocol How to Characterize Extracellular Vesicles by Western Blotting
- Tech Tip Acquiring DNA or RNA from Positively Selected Cells
- Technical Bulletin Evaluation of Genome Editing
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