Phalloidin is a heptapeptide derived from the poisonous mushroom Amanita phalloides. Phalloidin binds with a high specificity to F-actin filaments in cells, preventing filament depolymerization. Fluorescently labeled phalloidin can be used in labeling, identification, and quantitation of F-actin in formaldehyde-fixed and permeabilized cells. Fluorescently labeled phalloidin has lower non-specific binding compared to actin antibodies across most species of plants and animals, which provides low background and high contrast during imaging.
Data Figures
Figure 1. Phalloidin-iFluor™ Can Be Used to Stain Embryonic Stem Cells
H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).
Figure 2. Phalloidin-iFluor™ Can Be Used to Stain Forebrain-Type Neurons
WLS-1C cell-derived neural progenitor cells were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kits (Catalog #08605). Neurons were cultured on poly-L-ornithine/laminin-coated 35 mm glass-bottom dishes for 3 weeks, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages
Resources and Publications
Educational Materials (5)
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