WST-8 Cell Quantification Assay involves a simple, ready-to-use solution that can be used for the robust and high-throughput quantification of viable cell numbers. WST-8 Cell Quantification Assay solution can be directly added to cells and measured using a standard microplate reader within 1 - 4 hours. Mechanistically, this assay exploits the ability of the non-toxic tetrazolium salt, WST-8, to be reduced by cellular dehydrogenases, mediated by the electron carrier 1-methoxy-5-methylphenazinium methyl sulfate, into a water-soluble orange formazan dye. The amount of orange formazan product generated is directly proportional to the number of viable cells and can be quantified by measuring absorbance readings at 460 nm. Owing to the stability of the solution, this cell quantification assay can be used for protocols requiring longer incubation times ranging from 24 - 48 hours, with detection sensitivity being higher compared to the other tetrazolium salt-based assays, such as MTT, XTT or MTS.
Data Figures
Figure 1. WST-8 Cell Quantification Assay Shows Formazan Product Absorbance at 460 nm Is Directly Proportional to Cell Number
(A) HeLa cells (41-3333 cells) were plated in a clear-bottom 96-well plate with three-fold serial dilutions. (B) Jurkat cells (68-16,666 cells) were plated in a clear-bottom 96-well plate with three-fold serial dilutions. (A, B) Absorbance was measured at 460 nm after addition of WST-8 Cell Quantification Assay which shows a linear relationship between cell number and absorbance at 460 nm.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
WST-8 Cell Quantification Assay
Catalog #
100-1544
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
WST-8 Cell Quantification Assay
Catalog #
100-1544
Lot #
All
Language
English
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