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StemSpan™髓系扩增补充物II （100X）

无血清培养补充，扩大和分化的人单核细胞
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产品号 #02694_C

无血清培养补充，扩大和分化的人单核细胞

产品优势

  • 在14天的液体培养中，促进每个输入CD34+人CB细胞产生数百个CD14+单核细胞。
  • 作为100X浓缩物供应。解冻混合后，管内内容物可直接加入任何造血细胞扩增培养基中。
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概述

StemSpan™髓系扩增补充II （100X）含有重组人细胞因子的组合，用于选择性地促进人髓系祖细胞的扩增和分化，从人脐带血（CB）样本中分离的CD34+细胞中产生大量CD14+单核细胞。

StemSpan™髓系扩增补充II （100X）用于与以下任何StemSpan™培养基结合使用：
•StemSpan™SFEM（目录#09600）
StemSpan™SFEM II（目录#09605）
StemSpan™-XF（目录#100-0073）
StemSpan™-AOF（目录#100-0130）

当添加到无血清培养基中时，StemSpan™髓系扩增补充II通常促进每个输入CD34+细胞在CD34+人CB细胞启动的14天液体培养中产生数百个CD14+单核细胞。请参阅data选项卡了解更多详细信息。

Contains
• Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L)
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human macrophage colony-stimulating factor (M-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
• Supplements
 
Subtype
Supplements
 
Cell Type
Hematopoietic Stem and Progenitor Cells, Monocytes, Myeloid Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
StemSpan
 
Area of Interest
Stem Cell Biology, Transplantation Research
 
Formulation Category
Serum-Free
 

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Data Figures

Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II

Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement II (100X)
Catalog #
02694
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement II (100X)
Catalog #
02694
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Contains • Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L) • Recombinant human stem cell factor (SCF) • Recombinant human thrombopoietin (TPO) • Recombinant human macrophage colony-stimulating factor (M-CSF) • Recombinant human granulocyte/macrophage c
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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