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STEMdiff™ 神经嵴分化试剂盒

用于建立 hPSC 衍生神经嵴细胞的细胞培养试剂盒
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产品号 #（选择产品）

产品号 #08610_C

用于建立 hPSC 衍生神经嵴细胞的细胞培养试剂盒

产品优势

  • 快速：采用简单的单层培养方案，仅需六天即可生成神经嵴细胞
  • 高效：生成的多能神经嵴细胞纯度超过70%
  • 多能性：所生成的细胞具有多向分化能力，可进一步分化为外周神经元、软骨细胞和成骨细胞等
  • 多用途：兼容在 mTeSR™1、mTeSR™ Plus 或 TeSR™-E8™ 培养体系中维持的人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）

产品组分包括

  • STEMdiff™ 神经诱导培养基，250 mL（目录编号：05835）
  • STEMdiff™ 神经嵴分化补充剂，0.5 mL
main product photo
Need a high-quality cell source? Use the hiPSC SCTi003-A (female) or SCTi004-A (male) control lines, manufactured with mTeSR™ Plus.
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

STEMdiff™ 神经嵴分化试剂盒提供无血清培养基，使人胚胎干细胞（ES细胞）和诱导多能干细胞（iPS细胞）分化为神经嵴细胞。这些神经嵴细胞以 SOX10 和 CD271 等神经嵴标志物为特征，并可进一步分化为多种下游细胞类型，包括软骨细胞、成骨细胞和周围神经元。

该培养基兼容在以下培养体系中维持的人ES和iPS细胞：mTeSR™1（目录编号：85850）、mTeSR™ Plus（目录编号：100-0276）或 TeSR™-E8™（目录编号：05990）。

亚型
专用培养基
 
细胞类型
神经细胞，PSC衍生，神经干/祖细胞，神经元，多能干细胞
 
种属
人
 
应用
细胞培养，分化，细胞毒性检测
 
品牌
STEMdiff
 
研究领域
疾病建模，药物发现和毒理检测，神经科学，干细胞生物学
 
制剂类别
无血清
 

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Data Figures

Figure 1.

Neural crest cells are produced using STEMdiff™ Neural Crest Differentiation Kit after 6 days in culture. For information on continued passage of neural crest cells, contact us at techsupport@stemcell.com

STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

Figure 2. STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

After 6 days in culture, neural crest cells (NCCs; SOX10+, red; CD271+, light blue) outnumber CNS-type progenitors (PAX6+, green). (A) Channel merge of cells fixed 2 days after being passaged on day 6. Individual immunofluorescence channels show (B) DAPI, (C) PAX6, (D) SOX10, and (E) CD271. Scale bar = 100 μm.

NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

Figure 3. NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

NCCs (A) were cultured for 6 days and display typical morphology. (B) Culturing NCCs using established protocols generates peripheral neurons (Peripherin, green; BRN3a, red; DAPI, blue). (C) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™-ACF Chondrogenic Differentiation Kit generates a chondrocyte pellet (Alcian Blue, Nuclear Fast Red) with deposition of cartilage around the cells. (D) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™ Osteogenic Differentiation Kit (Human) generates an osteoblast culture with high levels of alkaline phosphatase-positive mineral deposition. Scale bar = 500 μm (A-C), 1 mm (D).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Neural Crest Differentiation Kit
Catalog #
08610
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
STEMdiff™ Neural Crest Differentiation Kit
Catalog #
08610
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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