STEMdiff™ 心肌细胞铺板补充剂

一种用于提高人多能干细胞衍生心肌细胞在复苏和收获后的铺板效率的专用培养试剂盒

产品号 #(选择产品)

产品号 #100-1120_C

一种用于提高人多能干细胞衍生心肌细胞在复苏和收获后的铺板效率的专用培养试剂盒

产品优势


  • Improve plating efficiency and double the number of hPSC-derived cardiomyocytes available for your experiments

  • Retain the stable electrical profile of hPSC-derived cardiomyocytes following replating

  • Save time and money with more efficient cardiomyocyte replating after thawing and dissociation

总览

Double the yield of human pluripotent stem cell (hPSC)-derived cardiomyocytes following dissociation, thawing, and replating by using the STEMdiff™ Cardiomyocyte Plating Kit. Consisting of the STEMdiff™ Cardiomyocyte Plating Supplement and STEMdiff™ Cardiomyocyte Support Medium, this kit improves plating efficiency and minimizes the loss of hPSC-derived cardiomyocytes.

This kit is designed for hPSC-derived cardiomyocytes generated using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit. By doubling your cardiomyocyte plating efficiency, the STEMdiff™ Cardiomyocyte Plating Kit can help increase your experimental throughput.

Use this kit as part of a complete, optimized workflow including these compatible STEMdiff™ cardiomyocyte products:

STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
STEMdiff™ Cardiomyocyte Dissociation Kit
STEMdiff™ Cardiomyocyte Support Medium
STEMdiff™ Cardiomyocyte Freezing Medium
STEMdiff™ Cardiomyocyte Maintenance Kit

包含
Serum
 
细胞类型
心肌细胞,PSC衍生
 
种属

 
应用
冻存,分化,培养
 
品牌
STEMdiff
 
研究领域
疾病建模,药物发现和毒理检测,干细胞生物学,细胞治疗开发
 

Data Figures

Graphs demonstrating iImproved plating: STEMdiff™ Cardiomyocyte Plating Medium (51%) vs. Support Medium (16%).

Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).

Microscope images showing enhanced attachment of replated hPSC-derived cardiomyocytes using plating medium.

Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit

Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.

Microelectrode array shows stable beat rate of cardiomyocytes replated with this kit.

Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile

Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1120
Lot #
All
Language
English
Catalog #
100-1120
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Contains Serum
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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