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RosetteSep™ HLA T细胞富集抗体混合物

免疫密度负选试剂混合物
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产品号 #（选择产品）

产品号 #15061HLA_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 分选得到的细胞未被标记

产品组分包括

  • RosetteSep™ HLA细胞富集抗体混合物（产品号 #15061HLA）
    • RosetteSep™ HLA T细胞富集抗体 混合物，5x2mL
  • RosetteSep™ HLA细胞富集抗体混合物（产品号 #15061HLA）
    • RosetteSep™ HLA T细胞富集抗体 混合物，20x2mL
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™人HLA T细胞富集抗体混合物设计通过负选从全血分离T细胞。四聚体抗体复合物可识别非T细胞和红细胞（RBC），从而靶向去除非目的细胞。使用密度梯度离心液（如RosetteSep™ DM-L（产品号 #15705）或Lymphoprep™（产品号 #07801）离心后 ，非目的细胞会与红细胞一起沉淀。纯化的T细胞为血浆和密度梯度离心液的交界界面中高度富集的细胞。 。获得的细胞可立即用于血清学、流式细胞术交叉配型检测或其他下游应用。

请注意：鉴于欧洲议会和理事会关于体外诊断医疗器械的新欧盟法规2017/746 (IVDR)，已于 2022年生效，STEMCELL取消了这些产品的CE IVD注册并删除了相关声明，现在这些产品标注为 "仅供研究使用"。请注意，这些产品的外形、功能和质量保证没有任何变化。

亚型
细胞分选试剂盒
 
细胞类型
T 细胞
 
种属
人
 
样本来源
Buffy Coat，Whole Blood
 
筛选方法
Negative
 
应用
细胞分选，体外诊断
 
品牌
RosetteSep
 
研究领域
HLA，免疫
 

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Data Figures

Typical RosetteSep™ HLA T Cell Enrichment Profile

Figure 1. Typical RosetteSep™ HLA T Cell Enrichment Profile

Starting with fresh whole blood the CD3+ cell content of the enriched fraction typically ranges from 90% - 97%. Red blood cells were removed by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
Lots above 1000081702
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
Lots above 1000081702
Language
Multi
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15081HLA
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15081HLA
Lot #
All
Language
MULTI
Document Type
Safety Data Sheet
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (5)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity
1:01:28
Webinar
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

Publications (1)

Provision of continuous maturation signaling to dendritic cells by RIG-I-stimulating cytosolic RNA synthesis of Sendai virus. Okano S et al. Journal of immunology (Baltimore, Md. : 1950) 2011 FEB

Abstract

Dendritic cell (DC)-based immunotherapy has potential for treating infections and malignant tumors, but the functional capacity of DC must be assessed in detail, especially maturation and Ag-specific CTL priming. Recent reports suggest that DC that are provided with continuous maturation signals in vivo after transfer into patients are required to elicit the full DC functions. We demonstrate in this study that the rSendai virus vector (SeV) is a novel and ideal stimulant, providing DC with a continuous maturation signal via viral RNA synthesis in the cytosol, resulting in full maturation of monocyte-derived DC(s). Both RIG-I-dependent cytokine production and CD4 T cell responses to SeV-derived helper Ags are indispensable for overcoming regulatory T cell suppression to prime melanoma Ag recognized by T cell-1-specific CTL in the regulatory T cell abundant setting. DC stimulated via cytokine receptors, or TLRs, do not show these functional features. Therefore, SeV-infected DC have the potential for DC-directed immunotherapy.
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更多信息

更多信息
Species Human
Sample Source Buffy Coat, Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD3抗体，克隆UCHT1
    抗人CD3抗体，克隆UCHT1

    小鼠（BALB/c）单克隆IgG1抗体，抗人、黑猩猩CD3

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ISO 14001 环境管理体系认证

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