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大鼠 IgM，kappa 同型对照抗体（克隆 RTK2118）

大鼠单克隆IgM， kappa同型对照抗体
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产品号 #60074_C

大鼠单克隆IgM， kappa同型对照抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

RTK2118 抗体 (IgM, kappa) 适合在多种应用中用作同型对照抗体，以评估抗原特异性抗体的非特异性结合程度。理想情况下，同型对照应与所用特异性抗体具有相同的重链亚类（IgA、IgD、IgE、IgG 或 IgM）和轻链亚类（kappa 或 lambda）。如果使用偶联抗体，则应选择与相同分子（例如荧光染料）偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性，并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。

该抗体克隆已验证可用作同型对照抗体，用于评估流式细胞术和免疫荧光显微镜应用（表面和细胞内染色）等应用中的细胞非特异性结合。

亚型
同型对照抗体
 
靶抗原
IgM Isotype Control
 
别名
Not applicable
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
其它细胞系
 
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
 
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
RTK2118
 
基因编号
Not applicable
 
同种型
IgM，kappa
 

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Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020), followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE. (C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631 (Catalog #60061), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118 followed by goat anti-mouse IgG, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488 (Catalog #60020AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, APC (Catalog #60020AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Biotin (Catalog #60020BT) followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, FITC (Catalog #60020FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, PE (Catalog #60020PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC. (C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE (Catalog #60061PE). Inset shows cells labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488
Catalog #
60074AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC
Catalog #
60074AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin
Catalog #
60074BT, 60074BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC
Catalog #
60074FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE
Catalog #
60074PE, 60074PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118
Catalog #
60074.1, 60074
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488
Catalog #
60074AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC
Catalog #
60074AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin
Catalog #
60074BT, 60074BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC
Catalog #
60074FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE
Catalog #
60074PE, 60074PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgM, kappa Isotype Control Antibody, Clone RTK2118
Catalog #
60074.1, 60074
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Human, Mouse, Non-Human Primate, Other, Rat
Clone RTK2118
Gene Id Not applicable
Alternative Names Not applicable
Isotype IgM, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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