小鼠IgM,kappa 同型对照抗体,克隆 MM-30

小鼠单克隆IgM, kappa同型对照抗体

产品号 #(选择产品)

产品号 #60069_C

小鼠单克隆IgM, kappa同型对照抗体

MM-30抗体(IgM, kappa)适合在多种应用中用作同型匹配的对照抗体,以评估抗原特异性抗体的非特异性结合程度。理想情况下,同型对照应具有与所用特异性抗体相同的重链亚类(IgA、IgD、IgE、IgG 或 IgM)和轻链亚类(kappa或lambda)。如果使用偶联抗体,则应选择与相同分子(如荧光染料)偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性以及可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。据报道,MM-30 抗体可识别海兔血蓝蛋白。

该抗体克隆已被验证可用作同种型对照抗体,用于评估流式细胞术和免疫荧光显微镜应用(表面和细胞内染色)等应用中与细胞的非特异性结合。

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA160 Antibody, Clone TRA-1-60R; Catalog #60064) followed by goat anti-mouse IgG, FITC (filled histogram). (B) Human H1 ES cells were cultured in mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-81 Antibody, Clone TRA-1-81; Catalog #60065).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488; Catalog #60064AD) (filled histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE; Catalog #60064PE) (filled histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60069PE.1, 60069PE
Lot #
All
Language
English
Catalog #
60069AD
Lot #
All
Language
English
Catalog #
60069
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60069PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60069PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60069AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60069
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

更多信息

更多信息
Species Human, Mouse, Non-Human Primate, Other, Rat
Clone MM-30
Gene Id Not applicable
Alternative Names Not applicable
Isotype IgM, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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