产品号 #60075_C
大鼠单克隆IgG1, kappa同型对照抗体
RTK2071 抗体 (IgG1, kappa) 适合在多种应用中用作同型对照抗体,以评估抗原特异性抗体的非特异性结合程度。理想情况下,同型对照应与所用特异性抗体具有相同的重链亚类(IgA、IgD、IgE、IgG 或 IgM)和轻链亚类(kappa 或 lambda)。如果使用偶联抗体,则应选择与相同分子(例如荧光染料)偶联的同型对照。使用合适的同型对照有助于确认抗原特异性抗体的特异性,并指示可能由与 Fc 受体或其他细胞成分结合引起的非特异性结合。
该抗体克隆已验证可用作同型对照抗体,用于评估流式细胞术和免疫荧光显微镜应用(表面和细胞内染色)等应用中的细胞非特异性结合。
亚型
同型对照抗体
靶抗原
IgG1 Isotype Control
别名
Not applicable
偶联
Alexa Fluor 488,APC,Biotin 或 生物素,FITC,PE,未偶联的
宿主物种
大鼠
细胞类型
其它细胞系
种属
人,小鼠,非人灵长类,其它细胞系,大鼠
应用
流式细胞术,功能学筛选,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,Western印迹
研究领域
免疫
克隆
RTK2071
基因编号
Not applicable
同种型
IgG1,kappa
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Figure 3. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 4. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.
Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human, Mouse, Non-Human Primate, Other, Rat |
---|---|
Clone | RTK2071 |
Gene Id | Not applicable |
Alternative Names | Not applicable |
Isotype | IgG1, kappa |
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