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ImmunoCult™小鼠Th1分化补充剂

补充小鼠naïve CD4+ T细胞向Th1细胞分化
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产品号 #10953_C

补充小鼠naïve CD4+ T细胞向Th1细胞分化

产品优势

  • 优化了从C57BL/6小鼠脾脏中分离的naïve CD4+ T细胞诱导Th1细胞的方法
  • 作为100X浓缩物供应；解冻和混合后，管内容物可直接添加到介质中
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. RPMI 1640 Medium
    RPMI 1640 Medium

    RPMI 1640 medium

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概述

ImmunoCult™小鼠Th1分化补充剂含有重组小鼠细胞因子和阻断单克隆抗体的组合，用于促进小鼠naïve CD4+ T细胞向Th1细胞的分化。该补充剂用于含有胎牛血清和其他添加剂的RPMI 1640培养基（目录#36750），以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂。

本产品推荐用于：
•小鼠naïve T细胞分化和Th1功能调控的研究
•开发将naïve T细胞向Th1亚群扩展和极化的程序

Contains
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant mouse interleukin 12 (IL-12)
• Rat anti-mouse interleukin 4 (anti-IL-4)
 
Subtype
Supplements
 
Cell Type
T Cells, T Cells, CD4+
 
Species
Mouse
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
ImmunoCult
 
Area of Interest
Immunology
 

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Data Figures

Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Mouse
Contains • Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4)
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