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ImmunoCult™ 人Th1分化添加剂

人naïve CD4+ T细胞向Th1细胞分化的无血清培养添加剂
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产品号 #10973_C

人naïve CD4+ T细胞向Th1细胞分化的无血清培养添加剂

产品优势

  • 专为诱导人Th1细胞分化而配制
  • 经优化可搭配使用ImmunoCult™-XF T细胞扩增培养基
  • 较竞品Th1分化试剂盒相比，体现更高占比的记忆T细胞
  • 较竞品Th1分化试剂盒相比，产IFN-γ的CD4+ T细胞的扩增数量高达1.6倍的
  • 以50倍浓缩液形式提供；解冻并混匀后，管内容物可直接添加至培养基中
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总览
实验数据
产品说明书及文档
应用领域
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总览

  • ImmunoCult™人Th1分化添加剂含有多种人源细胞因子和一种封闭抗体，旨在促进外周血来源的人初始CD4+T细胞高效稳定激活、扩增并分化为Th1细胞。ImmunoCult™人Th1分化添加剂可与ImmunoCult™-XF T细胞扩增培养基（产品号 #10981）和ImmunoCult™人CD3/CD28 T细胞激活剂（产品号 #10971）搭配使用。
  • 本产品推荐用于：
  • •研究人初始T细胞分化和Th1功能的调节
  • •开发初始T细胞扩增及向Th1亚群极化的工艺流程

包含
• Recombinant human interleukin 12 (IL-12) • Recombinant human interleukin 18 (IL-18) • Mouse anti-human interleukin 4 (anti-IL-4)
 
亚型
添加剂
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
人
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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Data Figures

Figure 1. ImmunoCult™ Human Th1 Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Th1 Differentiation Supplement Produces More Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973) in ImmunoCult™ and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype was significantly lower and the percentage of cells expressing a differentiated phenotype was significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p < 0.05; paired t-test on logit transformed data; n = 4).

Figure 3. Culture with ImmunoCult™ Human Th1 Differentiation Supplement Produces More IFNγ-Expressing Cells Than Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. After culture, differentiated CD4+ T cells (CD45RA-CD45RO+) were analyzed for IFNγ and IL-4 (not shown) expression, following stimulation with PMA and Ionomycin for 4 hours. A greater percentage of cells generated with ImmunoCult™ expressed intracellular IFNγ compared to those generated under competitor conditions, while no significant difference was found in the percentage of cells which expressed intracellular IL-4, with very low numbers of cells expressing IL-4 for both ImmunoCult™ and competitor. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p< 0.05; paired t-test on logit transformed data; n = 4).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Human Th1 Differentiation Supplement
Catalog #
10973
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Human Th1 Differentiation Supplement
Catalog #
10973
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Contains • Recombinant human interleukin 12 (IL-12) • Recombinant human interleukin 18 (IL-18) • Mouse anti-human interleukin 4 (anti-IL-4)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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