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GloCell™固定活力染料紫540

用于哺乳动物细胞活/死染色的胺标记荧光染料
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产品号 #（选择产品）

产品号 #75011_C

用于哺乳动物细胞活/死染色的胺标记荧光染料

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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

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概述

GloCell™Fixable Viability Dye Violet 540是一种荧光细胞活力染料，用于流式细胞术等应用中染色活/死哺乳动物细胞。染料不可逆地结合细胞内和细胞表面胺基团，可以在固定，渗透或冷冻保存之前使用。细胞膜受损的细胞可以渗透到GloCell™染料中，在细胞活力测定中，与活细胞相比，产生更大的荧光。


GloCell™Fixable Viability Dye Violet 540由405nm的紫色激光激发，其荧光发射最大值为540nm，可使用用于检测AmCyan的525/50带通滤光片检测。

Species
Human, Mouse, Non-Human Primate, Other, Rat
 
Application
Flow Cytometry, Immunofluorescence
 
Brand
GloCell
 
Area of Interest
Angiogenic Cell Research, Antibody Development, Cancer, Cell Line Development, Chimerism, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology, Epithelial Cell Biology, HIV, HLA, Hybridoma Generation, Immunology, Infectious Diseases, Metabolism, Mitosis, Neuroscience, Organoids, Respiratory Research, Stem Cell Biology, Transplantation Research, Cell Therapy Development
 

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Data Figures

Figure 1.

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 540. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 540.

Figure 2.

Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 540. Excitation max: 399 nm, Emission Max: 540 nm

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
GloCell™ Fixable Viability Dye Violet 540
Catalog #
75011.1, 75011
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
GloCell™ Fixable Viability Dye Violet 540
Catalog #
75011.1, 75011
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human, Mouse, Non-Human Primate, Other, Rat
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