产品号 #19954HLA_C
从经HetaSep™处理的全血中通过免疫磁珠负选分选不带标记的人B细胞
从经HetaSep™处理的全血中通过免疫磁珠负选分选不带标记的人B细胞
是用EasySep™ HLA B细胞富集: 用于处理全血的全套试剂盒,可简便高效地从经HetaSep™处理的全血或洗涤后的白细胞单采术样本中,通过免疫磁珠负选法分离高纯度的人B细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性,已在发表的研究中广泛应用超过20年。
在该EasySep™负选流程中,非目的细胞通过抗体复合物与磁珠标记。表达以下标志物的非目的细胞被靶向去除:CD2、CD3、CD14、CD16、CD33、CD36、CD41、CD43、CD56、CD66b及血型糖蛋白A(glyA)。使用EasySep™磁极吸附后,通过简单地将目的B细胞倾倒或吸取至一个新的试管中,即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。经磁珠分选后,目的B细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。
了解更多EasySep™免疫磁珠技术的工作原理,或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品,包括细胞鉴定、冻存等。
磁体兼容性
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
亚型
细胞分选试剂盒
细胞类型
B 细胞
种属
人
样本来源
Whole Blood
筛选方法
Negative
应用
细胞分选
品牌
EasySep,RoboSep
研究领域
嵌合体,HLA,免疫
Figure 1. Typical EasySep™ HLA B Cell Enrichment Profile
Starting with HetaSep™-treated whole blood, the CD19+ cell content of the enriched fraction typically ranges from 81.5 - 99.7% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.2 - 9.1 x 10e5 CD19+ cells are typically recovered.
Figure 2. FACS Data for Anti-Human CD19 Antibody, Clone HIB19, APC-Conjugated
Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA) and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC (Catalog # 60005AZ). Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
---|---|
Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000) |
Sample Source | Whole Blood |
Selection Method | Negative |
免疫密度负选试剂混合物
全血免疫磁珠负选试剂盒
冻存的人原代细胞
抗人、黑猩猩CD19的小鼠单克隆IgG1抗体
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