脐带血库

大量临床研究表明，CB 单位中的 CFU 总数是与中性粒细胞和血小板植入时间以及无关同种异体 CB 移植后总体生存率最相关的一个参数。1-6 ^查看 更多 Several regulatory bodies (e.g. FDA, AABB, NetCord-FACT) recommend or require that the number of CFUs in a CB unit be measured before cryopreservation and/or after thawing, prior to release of the unit to a transplant facility. Tools for standardization of the CFU assay include semi-solid methylcellulose-based medium, specialized cultureware and an instrument for automated counting of CFU assays. MethoCult™ methylcellulose-based medium is formulated to consistently stimulate the growth and differentiation of progenitor cells, while restricting the migration of progeny so that colonies are formed. SmartDish™ meniscus-free cultureware has been developed to allow for more accurate colony counting for manual or automated counting of CFU assays. By combining SmartDish™ with our instrument for automated colony counting and scoring, cord blood banks can ensure that their CFU assay results are more accurate and reproducible compared to those counted manually.

A new flow cytometry assay, the ALDHbr assay, was recently validated for its utility in measuring the frequency of viable, metabolically active HSPCs in CB.⁷ This assay combines the measurement of a cell’s aldehyde dehydrogenase (ALDH) activity with staining for cell surface marker expression and has been shown to correlate with CFU assay results.