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PBS-MINI MagDrive Bioreactor

Compact bioreactor with single-use vessels for high-throughput, 3D suspension culture of hPSCs and other cell types
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  • COMPACT. The compact, stainless steel Base Unit fits where you need it, even allowing operation inside incubators.

  • SAFE & EFFICIENT EXPANSION. The Vertical-Wheel™ impeller and U-shaped base create a low-shear mixing environment that is optimal for culturing hPSC aggregates of uniform size.

  • EASY AGGREGATE RECOVERY. The U-shaped base collects aggregates and the wide diameter cap provides easy access for aggregate recovery.

  • A PERFECT PAIR. The Base Unit is designed to be used with the 0.1 L and 0.5 L PBS-MINI MAG Single-Use Vessels, magnetically driving the vessels via MagDrive technology.

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    Reliably and rapidly scale up your 3D cell cultures and suspensions with the PBS-MINI MagDrive Bioreactor Base Unit and MAG Single-Use Vessels. The gentle yet efficient mixing provided by the Vertical-Wheel™ impeller enables the expansion of shear-sensitive cells without anti-foaming agents or shear protectants. Ideal for human pluripotent stem cells (hPSCs), the compact, sealed Base Unit can be used inside incubators. Conveniently control your culture system with a speed dial and digital display, and visualize cells in low-light conditions using built-in LED lights.

    PBS-MINI 0.1 L and 0.5 L MAG Single-Use Vessels are sold separately in packs of 4. These disposable vessels are animal component-free and meet the requirements for USP Class VI Testing for Plastics <88> and ISO 10993. PBS Biotech Inc. provides an 18-month warranty for the PBS-MINI MagDrive Bioreactor Base Unit, valid from date of shipment. This product is for Research Use Only.

    The PBS-MINI MagDrive Bioreactor Base Unit includes a power adapter that is compatible with Type A electrical outlets and 120V or 240V A/C inputs. A separate wall adaptor is needed for use outside North America; to request a region-specific power cord, please contact techsupport@stemcell.com.

    Data Figures

    Figure 1. Consistently High Daily Fold Expansion of Human Pluripotent Stem Cells (hPSCs) Is Achieved with the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

    Daily fold expansions of a human embryonic stem (ES) cell line (H9) and human induced pluripotent stem (iPS) cell line (WLS-1C), cultured in PBS-MINI (A) 0.1 and (B) 0.5 MAG Single-Use Vessels, show consistently high growth. The cells were seeded at a density of 8 x 104 viable cells/mL in 100 mL (for PBS-MINI 0.1 MAG) and 3 - 8 x 104 viable cells/mL in 500 mL (for PBS-MINI 0.5 MAG), and cultured for 4 days using mTeSR™3D (Catalog #03950). Fold expansion of each cell line was comparable to their growth in Nalgene™ filter bottles (Control), when seeded at a density of 1 x 105 viable cells/mL in 30 mL. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

    Figure 2. Culturing hPSCs in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Achieves High Cell Viability

    End-of-passage viabilities of a human ES cell line (H9) and human iPS cell line (WLS-1C) cultured in mTeSR™3D show that the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels support expansion of hPSCs at (A) 100 mL (with PBS-MINI 0.1 MAG) and (B) 500 mL (with PBS-MINI 0.5 MAG) culture volumes. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

    Figure 3. Markers of Undifferentiated hPSCs Harvested from the PBS-MINI 0.5 MAG Single-Use Vessels

    1C and H9 hPSC lines after 9 passages in the PBS-MINI 0.5 MAG Single-Use Vessels using mTeSR™3D show high OCT-4 and TRA-1-60 expression. Error bars are +/- SD (n = 1).

    Figure 4. Morphology of hPSC Aggregates Is Optimal When Using the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

    hPSCs, expanded in suspension using mTeSR™3D in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels, exhibit the typical morphological characteristics of hPSC aggregates in suspension culture, including a mostly spherical shape, clear edges (not smooth or shiny), an even color, and a dimpled or pock-marked appearance. Aggregates are typically 350-400 µm in size. Scale bar = 500 µm.

    Figure 5. PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Demonstrate Consistently High Daily Fold Expansion Compared to Common Alternative Culture Vessels

    Daily fold expansion of 7 cell lines in suspension culture vessels, tested from 2019 - 2022. Of the 9 vessels tested, those recommended for robust suspension culture scale-up are the Non Tissue Culture-Treated 6-Well Plate, Nalgene™ Filter Storage Bottle, PBS-MINI 0.1, and PBS-MINI 0.5. These vessels have a consistent mean daily fold expansion above 1.2.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    PBS-MINI MagDrive Bioreactor Base Unit
    Catalog #
    100-1005
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 1
    Product Name
    PBS-MINI 0.5 MAG Single-Use Vessel
    Catalog #
    100-1007
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 2
    Product Name
    PBS-MINI 0.5 MAG Single-Use Vessel
    Catalog #
    100-1007
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 1
    Product Name
    PBS-MINI 0.1 MAG Single-Use Vessel
    Catalog #
    100-1006
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 2
    Product Name
    PBS-MINI 0.1 MAG Single-Use Vessel
    Catalog #
    100-1006
    Lot #
    All
    Language
    English
    Document Type
    Technical Manual
    Product Name
    PBS-MINI MagDrive Bioreactor
    Catalog #
    100-1005
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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