产品号 #03950_C
用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基
cGMP, enzyme-free cell dissociation reagent
Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Compatible antibodies for purity assessment of isolated cells
mTeSR™3D用于未分化的人胚胎干细胞(ES)和人诱导多能干细胞(iPS)细胞在3D悬浮培养中的扩增和放大。新颖的进料批工作流程节省了时间和媒体。每日饲料补充营养,并消除非通过日媒介交换的需要。
mTeSR™3D是一种专门的无血清制剂。该培养系统与一系列悬浮培养容器兼容。
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation Category
Serum-Free
Figure 1. hPSC growth in mTeSR™3D
mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).
Figure 2. hPSC aggregate morphology in mTeSR3D
Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.
Figure 3. Expression of undifferentiated cell markers
hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.
Figure 4. Tri-lineage Differentiation
hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
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Formulation Category | Serum-Free |
通过人类胚胎干细胞和iPS细胞定向分化到所有三个胚层来评估多能性的功能测定试剂盒
冲洗培养皿的溶液以防止细胞粘附
小鼠抗人OCT4单克隆IgG2b抗体(OCT3)
cGMP,人类胚胎干细胞和iPS细胞的无饲料维持培养基
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