mTeSR™3 d

用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基

产品号 #(选择产品)

产品号 #03950_C

用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

概述

mTeSR™3D用于未分化的人胚胎干细胞(ES)和人诱导多能干细胞(iPS)细胞在3D悬浮培养中的扩增和放大。新颖的进料批工作流程节省了时间和媒体。每日饲料补充营养,并消除非通过日媒介交换的需要。

mTeSR™3D是一种专门的无血清制剂。该培养系统与一系列悬浮培养容器兼容。

Subtype
Specialized Media
 
Cell Type
Pluripotent Stem Cells
 
Species
Human
 
Application
Cell Culture, Expansion
 
Brand
TeSR
 
Area of Interest
Stem Cell Biology
 
Formulation Category
Serum-Free
 

Data Figures

Figure 1. hPSC growth in mTeSR™3D

mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

Figure 2. hPSC aggregate morphology in mTeSR3D

Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


Figure 3. Expression of undifferentiated cell markers

hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

Figure 4. Tri-lineage Differentiation

hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Formulation Category Serum-Free
Legal Statement: mTeSR™3D is manufactured and sold under global exclusive license from Accellta for culture medium for hPSCs in suspension under feeder-free, non-adherent conditions. This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。