重组小鼠SDF-1 α (CXCL12)

基质细胞衍生因子1α

产品号 #(选择产品)

产品号 #78121_C

基质细胞衍生因子1α

总览

基质细胞衍生因子1α (SDF-1α)是CXC趋化因子家族的一员,与G蛋白偶联受体CXCR4结合,调控多种细胞类型(包括造血干细胞、B细胞和T细胞)的迁移、增殖、分化和存活。它由骨髓基质细胞、成骨细胞、内皮细胞和神经元细胞产生。该因子首次在小鼠骨髓源性基质细胞系PA6中,被鉴定为B细胞前生长刺激因子(PBSF),因其能促进B细胞前体的生长 (Hayashi et al.)。SDF-1α主要在胚胎发育和成年期间的调控细胞运动,包括造血干细胞和中性粒细胞在胚胎发育过程中归巢到胎儿骨髓(Ara et al. 2003a)和成年时促血管生成过程中从骨髓募集内皮祖细胞(Zheng etal .)。除了调控造血外,SDF-1α/CXCR4信号通路对于原始生殖细胞向性腺的归巢(Ara et al. 2003b)、神经发生过程中小脑颗粒细胞的迁移(Zou et al.)以及乳腺癌细胞向转移部位的迁移(Muller et al.)也是必不可少的。

亚型
细胞因子
 
细胞类型
B 细胞,内皮细胞,上皮细胞,单核细胞,T 细胞
 
种属
小鼠
 
研究领域
内皮细胞研究,上皮细胞研究,免疫,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Mouse Recombinant SDF-1 alpha (CXCL12) was tested by its ability to mobilize Ca2+ in CHOK1/Gα15/hCXCR4 cells (human Gα15 and mCXCR4 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the above example is less than 1.5 μg/mL. (B) 2 μg of Mouse Recombinant SDF-1 alpha (CXCL12) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78121.1, 78121
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78121.1, 78121
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (1)

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Species Mouse
Purity ≥ 95%
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