产品号 #78121_C
基质细胞衍生因子1α
基质细胞衍生因子1α (SDF-1α)是CXC趋化因子家族的一员,与G蛋白偶联受体CXCR4结合,调控多种细胞类型(包括造血干细胞、B细胞和T细胞)的迁移、增殖、分化和存活。它由骨髓基质细胞、成骨细胞、内皮细胞和神经元细胞产生。该因子首次在小鼠骨髓源性基质细胞系PA6中,被鉴定为B细胞前生长刺激因子(PBSF),因其能促进B细胞前体的生长 (Hayashi et al.)。SDF-1α主要在胚胎发育和成年期间的调控细胞运动,包括造血干细胞和中性粒细胞在胚胎发育过程中归巢到胎儿骨髓(Ara et al. 2003a)和成年时促血管生成过程中从骨髓募集内皮祖细胞(Zheng etal .)。除了调控造血外,SDF-1α/CXCR4信号通路对于原始生殖细胞向性腺的归巢(Ara et al. 2003b)、神经发生过程中小脑颗粒细胞的迁移(Zou et al.)以及乳腺癌细胞向转移部位的迁移(Muller et al.)也是必不可少的。
亚型
细胞因子
细胞类型
B 细胞,内皮细胞,上皮细胞,单核细胞,T 细胞
种属
小鼠
研究领域
内皮细胞研究,上皮细胞研究,免疫,干细胞生物学
纯度
≥ 95 %
(A) The biological activity of Mouse Recombinant SDF-1 alpha (CXCL12) was tested by its ability to mobilize Ca2+ in CHOK1/Gα15/hCXCR4 cells (human Gα15 and mCXCR4 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the above example is less than 1.5 μg/mL. (B) 2 μg of Mouse Recombinant SDF-1 alpha (CXCL12) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
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Species | Mouse |
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Purity | ≥ 95% |
免疫磁珠正选试剂盒
冻存的人原代细胞
冻存的人原代细胞
冻存的人原代细胞
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