产品号 #78064_C
干细胞因子
干细胞因子 (SCF) 是一种早期作用的细胞因子,在胚胎和成人造血调控中发挥关键作用。SCF 促进细胞存活、增殖、分化、粘附以及在造血系统多个层面的功能激活。SCF 与其他细胞因子(例如血小板生成素和 Flt3/Flk-2 配体)一起,常用于促进培养的原始造血干细胞和多能祖细胞的扩增 (Huang et al.; Kent et al.)。SCF 与多种生长因子(包括 IL-2、IL-3、IL-6、IL-7、G-CSF 和促红细胞生成素)协同作用,促进髓系和红系祖细胞以及部分淋巴系祖细胞的增殖和分化 (Broudy)。在小鼠中,SCF 在胎儿性腺发育过程中至关重要 (Mauduit)。它由胎儿肝脏、骨髓和胸腺中的基质细胞、中枢神经系统、角质形成细胞和肠黏膜中的基质细胞产生,并可作为趋化因子和化学动力学因子发挥作用。
SCF 有两种生物活性剪接形式:可溶性剪接体和跨膜剪接体。与其受体(c-Kit 酪氨酸激酶受体;CD117)结合后,SCF 可激活 PI3K、JAK/STAT 和 MAPK 通路。据报道,SCF 和 c-Kit 信号传导在色素沉着、生育力、血管生成、通过 c-Kit 阳性的 Cajal 间质细胞促进肠道运动以及神经干细胞和祖细胞向脑损伤部位迁移方面发挥重要作用(Lennartsson & Ronnstrand)。
亚型
细胞因子
细胞类型
造血干/祖细胞,中胚层,PSC衍生,多能干细胞
种属
小鼠
研究领域
干细胞生物学
纯度
≥ 95 %
(A) The biological activity of Mouse Recombinant SCF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 65 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 5.7 - 8.6 ng/mL.
(B) 1 μg of Mouse Recombinant SCF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant SCF has a predicted molecular mass of 18.4 kDa.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Species | Mouse |
---|---|
Purity | ≥ 95% |
粒细胞集落刺激因子
白介素3
白介素6
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