产品号 #78064_C
Stem cell factor
概述
Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Huang et al.; Kent et al.). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). In the mouse, SCF is essential during fetal gonadal development (Mauduit). It is produced by stromal cells in the fetal liver, bone marrow, and thymus, in the central nervous system, in keratinocytes, and in the gut mucosa, and can function as a chemotactic and chemokinetic factor.
SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit has also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit-positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain (Lennartsson & Ronnstrand).
Subtype
Cytokines
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Mouse
Area of Interest
Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Mouse Recombinant SCF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 65 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 5.7 - 8.6 ng/mL.
(B) 1 μg of Mouse Recombinant SCF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant SCF has a predicted molecular mass of 18.4 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mouse Recombinant SCF (E. coli-expressed) Catalog #
78064.1, 78064, 78064.2, 78064.3 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Mouse Recombinant SCF (E. coli-expressed) Catalog #
78064.1, 78064, 78064.2, 78064.3 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Mouse Pluripotent Stem Cell Research
Resources and Publications
Educational Materials (1)
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