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ImmunoCult™ 人B细胞扩增试剂盒

扩增B细胞的无血清培养试剂盒
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产品号 #（选择产品）

产品号 #100-0645_C

扩增B细胞的无血清培养试剂盒

产品优势

  • 无需使用血清、饲养细胞或专用培养板，即可在体外高效扩增人类B细胞
  • 无血清培养基和无动物源成分添加剂

产品组分包括

  • ImmunoCult™-XF B细胞基础培养基，100ml；
  • ImmunoCult™-ACF人B细胞扩增添加剂，2ml
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

无需使用血清、饲养层细胞或专用培养板，即可实现人B细胞的稳定扩增用于下游检测和应用。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

亚型
专用培养基，添加剂
 
细胞类型
B 细胞
 
种属
人
 
应用
细胞培养
 
品牌
ImmunoCult
 
研究领域
抗体​制备，药物发现和毒理检测，免疫，感染性疾病(传染病)，干细胞生物学，细胞治疗开发
 
制剂类别
无血清，Xeno-Free
 

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Data Figures

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Human B Cell Expansion Kit
Catalog #
100-0645
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free, Xeno-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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