切换导航
  • 比较产品
我的购物车

ImmunoCult™-ACF Human B Cell Expansion Supplement

Serum-free and animal component-free culture supplement for expansion of human B cells
率先评论此产品
添加并比较

产品号 #10974_C

Serum-free and animal component-free culture supplement for expansion of human B cells

产品优势


  • Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates

  • Serum-free and animal component-free supplement

产品组分包括

  • ImmunoCult™-ACF Human B Cell Expansion Supplement, 2 mL
main product photo
ImmunoCult™-ACF Human B Cell Expansion Supplement
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. ImmunoCult™-XF B Cell Base Medium
    ImmunoCult™-XF B Cell Base Medium

    Serum-free and xeno-free base medium for the expansion of B cells

概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

ImmunoCult™-ACF Human B Cell Expansion Supplement is a serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.

Available either individually or as part of ImmunoCult™ Human B Cell Expansion Kit. ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF B Cell Base Medium to prepare a complete serum-free medium for culturing and expanding human B cells.

Contains
<div> Recombinant proteins, including CD40L and other cytokines </div> <br> <div> Chemically defined components
 
Subtype
Supplements
 
Cell Type
B Cells
 
Species
Human
 
Application
Activation, Cell Culture, Differentiation, Expansion, Genome Editing
 
Brand
ImmunoCult
 
Area of Interest
Antibody Development, Immunology, Infectious Diseases
 
Formulation Category
Animal Component-Free, Serum-Free
 

Data Figures

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。