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ImmunoCult™-ACF 人B细胞扩增添加物

无血清、无动物成分的培养添加物，用于人B细胞扩增
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产品号 #（选择产品）

产品号 #10974_C

无血清、无动物成分的培养添加物，用于人B细胞扩增

产品优势

  • 无需血清、饲养层细胞或专用培养板即可实现人B细胞的强力体外扩增
  • 无血清、无动物成分的添加物。

产品组分包括

  • ImmunoCult™-ACF 人B细胞扩增添加物，2 mL
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

ImmunoCult™-ACF 人B细胞扩增添加物是一种无血清且无动物成分的添加物，专为在无血清、饲养层细胞或专用培养板的条件下促进人外周血B细胞的强力体外扩增而设计。该添加物含有重组蛋白和化学上成分明确的组分，可支持人B细胞的活化、扩增及其向浆细胞的成熟。

该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分，也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物，可添加至基础培养基（如ImmunoCult™-XF B细胞基础培养基）中，配制成用于培养和扩增人B细胞的无血清完全培养基。

包含
重组蛋白，including CD40L and other cytokines Chemically defined components
 
亚型
添加剂
 
细胞类型
B 细胞
 
种属
人
 
应用
激活，细胞培养，分化，扩增，基因组编辑
 
品牌
ImmunoCult
 
研究领域
抗体​制备，免疫，感染性疾病(传染病)
 
制剂类别
Animal Component-Free，无血清
 

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Data Figures

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Contains Recombinant proteins, including CD40L and other cytokines Chemically defined components
Formulation Category Animal Component-Free, Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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