产品号 #78068_C
肿瘤坏死因子
肿瘤坏死因子-α (TNF-α) 是一种促炎细胞因子,可激活 NF-kB、MAPK 和 PI3K/AKT 通路。活化的 T 细胞和巨噬细胞是炎症和感染条件下 TNF-α 的主要产生者。许多其他类型的细胞已被证实也能产生 TNF-α,其中包括 B 细胞、NK 细胞、肥大细胞、中性粒细胞、树突状细胞、小胶质细胞、内皮细胞、平滑肌细胞、心肌细胞和成纤维细胞。TNF-α 通过刺激抗肿瘤免疫抑制反应,在体外对癌细胞产生细胞毒作用。TNF-α 刺激 E 选择素和 P 选择素的表达,从而促进中性粒细胞、单核细胞和记忆 T 细胞粘附于活化的血小板和内皮细胞 (Zelová & Hosek)。TNF-α 的其他作用包括血管扩张和水肿形成。
亚型
细胞因子
细胞类型
气道细胞,B 细胞,造血干/祖细胞,肠道细胞,神经元,NK 细胞
种属
人
研究领域
上皮细胞研究,免疫,神经科学,干细胞生物学
纯度
≥ 95 %
(A) The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.4 0.6 ng/mL.
(B) 1 μg of Human Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TNF-alpha has a predicted molecular mass of 17.5 kDa.
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Species | Human |
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Purity | ≥ 95% |
白介素1β
白介素6
肿瘤坏死因子
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