重组人TNF - α

肿瘤坏死因子

产品号 #(选择产品)

产品号 #78068_C

肿瘤坏死因子

总览

肿瘤坏死因子-α (TNF-α) 是一种促炎细胞因子,可激活 NF-kB、MAPK 和 PI3K/AKT 通路。活化的 T 细胞和巨噬细胞是炎症和感染条件下 TNF-α 的主要产生者。许多其他类型的细胞已被证实也能产生 TNF-α,其中包括 B 细胞、NK 细胞、肥大细胞、中性粒细胞、树突状细胞、小胶质细胞、内皮细胞、平滑肌细胞、心肌细胞和成纤维细胞。TNF-α 通过刺激抗肿瘤免疫抑制反应,在体外对癌细胞产生细胞毒作用。TNF-α 刺激 E 选择素和 P 选择素的表达,从而促进中性粒细胞、单核细胞和记忆 T 细胞粘附于活化的血小板和内皮细胞 (Zelová & Hosek)。TNF-α 的其他作用包括血管扩张和水肿形成。

亚型
细胞因子
 
细胞类型
气道细胞,B 细胞,造血干/祖细胞,肠道细胞,神经元,NK 细胞
 
种属

 
研究领域
上皮细胞研究,免疫,神经科学,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.4 0.6 ng/mL.
(B) 1 μg of Human Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TNF-alpha has a predicted molecular mass of 17.5 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78068.1, 78068
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78068.1, 78068
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

更多信息

更多信息
Species Human
Purity ≥ 95%
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