重组人干扰素γ

干扰素-γ

产品号 #(选择产品)

产品号 #78020_C

干扰素-γ

总览

干扰素-γ (IFN-γ),也称为 II 型干扰素,由 T 细胞和 NK 细胞产生,树突状细胞和巨噬细胞也产生少量干扰素。IFN-γ 受感染后分泌的细胞因子例如 IL-12 和 IL-18控制(Schroder et al.)。IFN-γ 与受体复合物结合,并通过 JAK/STAT 通路启动信号转导;最终导致许多基因的转录和激活,这些基因控制着多种免疫功能(de Weerd & Nguyen; Krause et al.)。IFN-γ 通过促进微生物效应功能的激活,例如活性氧、一氧化氮和补体的产生(Schroder et al.),刺激巨噬细胞、NK 细胞和中性粒细胞的抗菌和抗肿瘤活性(Billiau & Matthys)。 IFN-γ 可增强树突状细胞和单核吞噬细胞中 MHC I 类和 II 类分子的表达,并促进树突状细胞产生 IL-12。在 B 细胞中,IFN-γ 可刺激小鼠和人细胞的存活和生长,并使 B 细胞从增殖转向分化。IFN-γ 有利于 Th1 细胞而非 Th2 细胞的发育,并刺激单核细胞分化和功能(Schroder et al.)。

亚型
细胞因子
 
细胞类型
B 细胞,造血干/祖细胞,肠道细胞,间充质干/祖细胞,T 细胞
 
种属

 
研究领域
上皮细胞研究,免疫,干细胞生物学
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Human Recombinant IFN-gamma was tested by its ability to promote the cytotoxicity of HT-29 (HTB-38) cells. Cytotoxicity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell death is at 50% of maximum. The EC50 for this effect is 0.035 ng/mL.
(B) 2 μg of Human Recombinant IFN-gamma was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma has a predicted molecular mass of 17 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78020.1, 78020.2, 78020
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78020.1, 78020.2, 78020
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

更多信息

更多信息
Species Human
Purity ≥ 95%
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