产品号 #78027_C
Interleukin 12
概述
Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann & Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation, and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).
Subtype
Cytokines
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, NK Cells, T Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Purity
≥ 95%
Data Figures
(A) The biological activity of Human Recombinant IL-12 was tested by its ability to produce IFN-gamma in NK cells co-stimulated with IL18. The EC50 is defined as the effective concentration of the growth factor at which IFN-gamma production is at 50% of maximum. The EC50 in the above example is less than 2 ng/mL.
(B) 2 μg of Human Recombinant IL-12 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-12 has a predicted molecular mass of 22.5 - 34.7 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant IL-12 Catalog #
78027.2 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Human Recombinant IL-13 Catalog #
78027 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Human Recombinant IL-14 Catalog #
78027.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant IL-12 Catalog #
78027.2 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant IL-13 Catalog #
78027 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant IL-14 Catalog #
78027.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for T Cell Research
Workflow Stages for T Cell Engineering
Resources and Publications
Educational Materials (3)
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