产品号 #78141_C
干扰素- γ,不含动物成分
干扰素γ (IFN-γ),也被称为II型干扰素,由T细胞和NK细胞产生,少量由树突状细胞和巨噬细胞产生。IFN-γ由应对感染而分泌的细胞因子(例如IL-12和IL-18)控制(Schroder et al.)。IFN-γ结合受体复合物并通过JAK/STAT通路启动信号转导;这最终导致许多基因的转录和激活,这些基因控制着多种免疫功能(de Weerd and Nguyen; Krause et al.)。IFN-γ通过促进微生物效应功能的激活,如活性氧、NO中间体和补体的产生(Schroder et al.),刺激巨噬细胞、NK细胞和中性粒细胞的抗菌和抗肿瘤活性(Billiau & Matthys)。IFN-γ可增强树突状细胞和单核吞噬细胞中MHC I类和II类的表达,并促进树突状细胞产生IL-12。在B细胞中,IFN-γ刺激小鼠和人细胞的存活和生长,并将B细胞从增殖转向分化。IFN-γ可促进Th1细胞的发育,同时抑制Th2细胞形成,并刺激单核细胞分化和功能(Schroder et al.)。本产品不含动物成分。
亚型
细胞因子
别名
干扰素 gamma;II 型干扰素
细胞类型
B 细胞,造血干/祖细胞,肠道细胞,间充质干/祖细胞,T 细胞
种属
人
研究领域
上皮细胞研究,免疫,干细胞生物学
制剂类别
Animal Component-Free
纯度
≥ 95 %
Figure 1. Biological Activity and Molecular Weight of Human Recombinant IFN-gamma, ACF
(A) The biological activity of Human Recombinant IFN-gamma, ACF was measured with A549 cells exposed to the encephalomyocarditis (EMC) virus in a cytopathic effect (CPE) assay. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the example above is ≤ 0.75 ng/mL. (B) 1 μg of Human Recombinant IFN-gamma, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma, ACF has a predicted molecular mass of 16.9 kDa.
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Species | Human |
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Formulation Category | Animal Component-Free |
Alternative Names | Interferon gamma, Type II interferon |
Purity | ≥ 95% |
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