人重组IFN - γ, ACF

干扰素- γ,不含动物成分

产品号 #(选择产品)

产品号 #78141_C

干扰素- γ,不含动物成分

总览

干扰素γ (IFN-γ),也被称为II型干扰素,由T细胞和NK细胞产生,少量由树突状细胞和巨噬细胞产生。IFN-γ由应对感染而分泌的细胞因子(例如IL-12和IL-18)控制(Schroder et al.)。IFN-γ结合受体复合物并通过JAK/STAT通路启动信号转导;这最终导致许多基因的转录和激活,这些基因控制着多种免疫功能(de Weerd and Nguyen; Krause et al.)。IFN-γ通过促进微生物效应功能的激活,如活性氧、NO中间体和补体的产生(Schroder et al.),刺激巨噬细胞、NK细胞和中性粒细胞的抗菌和抗肿瘤活性(Billiau & Matthys)。IFN-γ可增强树突状细胞和单核吞噬细胞中MHC I类和II类的表达,并促进树突状细胞产生IL-12。在B细胞中,IFN-γ刺激小鼠和人细胞的存活和生长,并将B细胞从增殖转向分化。IFN-γ可促进Th1细胞的发育,同时抑制Th2细胞形成,并刺激单核细胞分化和功能(Schroder et al.)。本产品不含动物成分。

亚型
细胞因子
 
别名
干扰素 gamma;II 型干扰素
 
细胞类型
B 细胞,造血干/祖细胞,肠道细胞,间充质干/祖细胞,T 细胞
 
种属

 
研究领域
上皮细胞研究,免疫,干细胞生物学
 
制剂类别
Animal Component-Free
 
纯度
≥ 95 %
 

Data Figures

Figure 1. Biological Activity and Molecular Weight of Human Recombinant IFN-gamma, ACF

(A) The biological activity of Human Recombinant IFN-gamma, ACF was measured with A549 cells exposed to the encephalomyocarditis (EMC) virus in a cytopathic effect (CPE) assay. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the example above is ≤ 0.75 ng/mL. (B) 1 μg of Human Recombinant IFN-gamma, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma, ACF has a predicted molecular mass of 16.9 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78141.1, 78141.3
Lot #
1000168082 and lower
Language
English
Catalog #
78141.1, 78141.3
Lot #
1000168083 and higher
Language
English
Document Type
Safety Data Sheet
Catalog #
78141.1, 78141.3
Lot #
All
Language
English

更多信息

更多信息
Species Human
Formulation Category Animal Component-Free
Alternative Names Interferon gamma, Type II interferon
Purity ≥ 95%
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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