人/小鼠重组BMP-2

骨形态发生蛋白2,不含动物成分

产品号 #(选择产品)

产品号 #78135_C

骨形态发生蛋白2,不含动物成分

总览

骨形态发生蛋白2(BMP-2)是转化生长因子β (TGF-β) 超家族的一员。BMP-2是一种二硫键连接的同型二聚体,作为I型和II型BMP受体复合物的配体,主要激活SMAD1/5/8信号传导(Nohe et al.)。BMP-2是一种强效的分化因子,可引导人多能干细胞(hPSCs)分化为各种细胞类型,包括胚胎外内胚层、间充质细胞和软骨细胞(Pera et al.)。尽管BMP-2在健康软骨中的表达较低,但其在软骨损伤部位的表达上调(Blaney Davidson et al.)。BMP-2在体外诱导骨和软骨形成,并能诱导人间充质干细胞分化成软骨(Schmitt et al.)。本产品不含动物成分。

亚型
细胞因子
 
细胞类型
内胚层,PSC衍生,造血干/祖细胞,中胚层,PSC衍生,多能干细胞
 
种属
人,小鼠
 
研究领域
上皮细胞研究,神经科学,干细胞生物学
 
制剂类别
Animal Component-Free
 
纯度
≥ 95 %
 

Data Figures

(A) The biological activity of Human/Mouse Recombinant BMP-2, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the example above is 74 ng/mL. (B) 1 μg of Human/Mouse Recombinant BMP-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78135.2, 78135, 78135.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78135.2, 78135, 78135.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human, Mouse
Formulation Category Animal Component-Free
Purity ≥ 95%
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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