产品号 #78135_C
骨形态发生蛋白2,不含动物成分
骨形态发生蛋白2(BMP-2)是转化生长因子β (TGF-β) 超家族的一员。BMP-2是一种二硫键连接的同型二聚体,作为I型和II型BMP受体复合物的配体,主要激活SMAD1/5/8信号传导(Nohe et al.)。BMP-2是一种强效的分化因子,可引导人多能干细胞(hPSCs)分化为各种细胞类型,包括胚胎外内胚层、间充质细胞和软骨细胞(Pera et al.)。尽管BMP-2在健康软骨中的表达较低,但其在软骨损伤部位的表达上调(Blaney Davidson et al.)。BMP-2在体外诱导骨和软骨形成,并能诱导人间充质干细胞分化成软骨(Schmitt et al.)。本产品不含动物成分。
亚型
细胞因子
细胞类型
内胚层,PSC衍生,造血干/祖细胞,中胚层,PSC衍生,多能干细胞
种属
人,小鼠
研究领域
上皮细胞研究,神经科学,干细胞生物学
制剂类别
Animal Component-Free
纯度
≥ 95 %
(A) The biological activity of Human/Mouse Recombinant BMP-2, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the example above is 74 ng/mL. (B) 1 μg of Human/Mouse Recombinant BMP-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Species | Human, Mouse |
---|---|
Formulation Category | Animal Component-Free |
Purity | ≥ 95% |
激活素A,不含动物成分
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血管内皮生长因子,不含动物成分
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