The Human Interleukin 5 (IL-5) ELISA Kit is designed for the quantitative detection and measurement of human IL-5 in biological fluids such as serum, plasma, and cell culture supernatants. IL-5 is produced by Th2 cells, eosinophils, and activated mast cells. IL-5 plays an important role in the recruitment, activation, proliferation, and survival of eosinophils.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype Complete Kits
Cell Type Granulocytes and Subsets, T Cells, Other Subsets
Species Human
Application ELISA
Area of Interest Immunology
Data Figures
Representative Standard Curve
• Reportable range: 10 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. • Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 90/586. • Recovery: A mid-curve recovery of 92 - 110% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 1.6% (CV). The inter-assay precision of this assay is 12.0% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
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