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RosetteSep™DM-L密度介质

密度梯度介质
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产品号 #15705_C

密度梯度介质，与RosetteSep™ CD3+ T细胞、CD4+ T细胞、CD8+ T细胞和B细胞富集抗体混合物搭配使用

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RosetteSep™DM-L是一种密度梯度介质，专为与RosetteSep™抗体混合物搭配使用而设计，用于从全血中富集特定的人淋巴细胞群（CD3+ T 细胞、CD4+ T 细胞、CD8+ T 细胞和 B 细胞）。RosetteSep™ DM-L 的密度为 1.081 g/mL。

请注意：根据欧洲议会和欧盟理事会关于体外诊断医疗器械的新法规 2017/746 (IVDR)，该法规预计将于 2022 年生效，STEMCELL 近期已注销并取消了与这些产品相关的 CE IVD 声明，这些产品现已标记为“仅供研究使用”。请注意，这些产品的形式、功能和质量保证均未发生变化。如需了解更多信息，请联系质量保证和监管部门，邮箱：qaschangenote@stemcell.com。
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Data Figures

Figure 1. Minimal Granulocyte Contamination Using RosetteSep™ DM-L Density Medium

Figure 1. Minimal Granulocyte Contamination Using RosetteSep™ DM-L Density Medium

Granulocyte contamination is typically less than 1% when enriching lymphocytes from fresh whole blood using RosetteSep™. There was no significant difference in the level of granulocyte contamination when using RosetteSep™ DM-L in place of Ficoll-paque PLUS to enrich lymphocytes (N=11)

Rosettesep™ DM-L Improves the Recovery of Lymphocytes Enriched with Rosettesep™ from Fresh Blood

Figure 2. Rosettesep™ DM-L Improves the Recovery of Lymphocytes Enriched with Rosettesep™ from Fresh Blood

Cell Purity Is Equivalent or Higher for Rosettesep™ Lymphocyte Enrichment Using Rosettesep™ DM-L Instead of Ficoll-Paque

Figure 3. Cell Purity Is Equivalent or Higher for Rosettesep™ Lymphocyte Enrichment Using Rosettesep™ DM-L Instead of Ficoll-paque®

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
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Document Type
Product Information Sheet 1
Product Name
RosetteSep™ DM-L Density Medium
Catalog #
15705
Lot #
Lots above 1000053496
Language
Multi
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ DM-L Density Medium
Catalog #
15705
Lot #
Lots above 1000053496
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ DM-L Density Medium
Catalog #
15705
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Resources and Publications

Educational Materials (3)

Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...

Frequently Asked Questions

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

Publications (1)

Completely phased genome sequencing through chromosome sorting. Yang H et al. Proceedings of the National Academy of Sciences of the United States of America 2011 JAN

Abstract

The two haploid genome sequences that a person inherits from the two parents represent the most fundamentally useful type of genetic information for the study of heritable diseases and the development of personalized medicine. Because of the difficulty in obtaining long-range phase information, current sequencing methods are unable to provide this information. Here, we introduce and show feasibility of a scalable approach capable of generating genomic sequences completely phased across the entire chromosome.
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Species Human
Contains • Iodixanol • Hetastarch • Sodium chloride • Sodium lactate • Dextrose • Calcium chloride • Potassium chloride • Magnesium chloride • Water
Sample Source Buffy Coat, Whole Blood
Selection Method Negative
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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