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hPSC基因检测试剂盒

qPCR 分析试剂盒，用于检测人胚胎干细胞（ES）和诱导多能干细胞（iPS）中多数已报道的核型异常。
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产品号 #07550_C

qPCR 分析试剂盒，用于检测人胚胎干细胞（ES）和诱导多能干细胞（iPS）中多数已报道的核型异常。

产品组分包括

  • qPCR Master Mix (2X), 3 mL
  • ROX参比染料, 0.2 mL
  • Chr 1q 基因检测试剂, 60次反应量(Rxn)
  • Chr 4p 基因检测试剂, 60次反应量(Rxn)
  • Chr 8q 基因检测试剂, 60次反应量(Rxn)
  • Chr 10p 基因检测试剂, 60次反应量(Rxn)
  • Chr 12p 基因检测试剂, 60次反应量(Rxn)
  • Chr 17q 基因检测试剂, 60次反应量(Rxn)
  • Chr 18q 基因检测试剂, 60次反应量(Rxn)
  • Chr 20q 基因检测试剂, 60次反应量(Rxn)
  • Chr Xp 基因检测试剂, 60次反应量(Rxn)
  • 基因组DNA对照, 15 µL
  • TE缓冲液, 1 mL
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总览
实验数据
产品说明书及文档
应用领域
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总览

快速、经济地检测人多能干细胞（hPSCs）中报告最常见的8种核型异常。

该qPCR试剂盒可用于对多个hPSC株系进行遗传筛查，包含的试剂量足以对20个独立样本进行三重复检测。该试剂盒采用双猝灭探针，配有5-羧基荧光素（5-FAM）染料，相较于传统的单猝灭探针，具有更优异的性能。试剂盒内含qPCR Master Mix、ROX参比染料、缓冲液，以及用于检测8个最常发生突变区域关键最小区域的引物/探针混合液，同时还包含用于基因组中对照位点的引物/探针混合液。试剂盒还提供一份基因组DNA对照样本，其在所关注区域中已验证具有正常的拷贝数。

要了解您的结果，请将qPCR数据输入我们的在线 Genetic Analysis App（遗传分析应用程序）。该应用程序将执行统计分析、辅助数据解读，并提供数据的可视化呈现。

细胞类型
多能干细胞
 
种属
人
 
应用
基因组编辑
 
研究领域
疾病建模，药物发现和毒理检测，干细胞生物学
 

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Data Figures

Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy

Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation

Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication

Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).

Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism

Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
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Product Information Sheet
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 3
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 4
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 5
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 6
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 7
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 8
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 9
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 10
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 11
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 12
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 13
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (37)

Detect Karyotypic Abnormalities in Human ES & iPS Cells
Brochure
Detect Karyotypic Abnormalities in Human ES & iPS Cells
hPSC-Derived Cell Therapy Workflow
Brochure
hPSC-Derived Cell Therapy Workflow
hPSC Genetic Analysis Kit Technical Tips & Tricks
Technical Bulletin
hPSC Genetic Analysis Kit Technical Tips & Tricks
How to Use the hPSC Genetic Analysis Kit with 96-Well Plates
Protocol
How to Use the hPSC Genetic Analysis Kit with 96-Well Plates
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
1:29
Video
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
5:20
Video
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
8:33
Video
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
7:57
Video
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
3:35
Video
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
10:08
Webinar
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
17:30
Webinar
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing eTeSR™
33:00
Webinar
Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing ...
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussion
31:25
Webinar
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussio...
From hPSCs to Immune Cells: Mechanisms of Selection in Human Pluripotent Stem Cell Cultures
28:31
Webinar
From hPSCs to Immune Cells: Mechanisms of Selection in Human Pluripotent Stem Cell Cultures
Maintaining and Assessing High-Quality hPSC Cultures
55:53
Webinar
Maintaining and Assessing High-Quality hPSC Cultures
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
17:45
Webinar
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Considerations for Clinical-Grade iPSCs
47:23
Webinar
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Co...
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
13:01
Webinar
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
42:10
Webinar
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenanc...
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
29:03
Webinar
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
Consequences of Culture-Acquired Genetic Changes in Human Pluripotent Stem Cells
57:26
Webinar
Consequences of Culture-Acquired Genetic Changes in Human Pluripotent Stem Cells
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
19:35
Webinar
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
18:48
Webinar
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
50:01
Webinar
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Nature Research Round Table: Pluripotency Tests
17:46
Webinar
Nature Research Round Table: Pluripotency Tests
Comparative Study: Improving the Genetic Stability of Single-Cell-Passaged Human Pluripotent Stem Cell Cultures Using eTeSR™
22:32
Webinar
Comparative Study: Improving the Genetic Stability of Single-Cell-Passaged Human Pluripotent Stem Ce...
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
24:46
Webinar
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Re-Creating Disease with Kidney Organoids and CRISPR
1:14:36
Webinar
Re-Creating Disease with Kidney Organoids and CRISPR
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
15:47
Webinar
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
13:58
Webinar
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
1:13:44
Webinar
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells
16:56
Webinar
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripote...
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
21:35
Webinar
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration

更多信息

更多信息
Species Human
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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