产品号 #100-0385_C
人类肝类器官长期扩张和维持的培养基
Reagent for counting viable mammalian cells
Reagent for lysis of red blood cells
Clear polystyrene flat-bottom, tissue culture-treated multiwell cell culture plate with lid
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Compatible antibodies for purity assessment of isolated cells
长期可靠地扩展和维持人类肝脏类器官,作为肝脏生物学研究、疾病建模、药物发现和治疗筛选的模型系统。使用hepatcult™类器官生长培养基(OGM)(人)从人肝组织或psc衍生的肝细胞中培养肝类器官STEMdiff™肝细胞检测试剂盒.
可用的单独可用或作为…的一部分可用的hepatcult™类器官试剂盒(人), hepatcult™OGM可用于培养和扩增肝类器官,用于未来的实验或生物银行。类器官hepatcult™类器官起始培养基(人)并扩展到表达自我更新(LGR5, AXIN2),导管(KRT19, SOX9, KRT7)和肝祖细胞(HNF4A)标记的hepatcult™OGM中。这些类器官也可以用hepatcult™类器官分化培养基(人)成熟的类器官显示肝脏功能,包括CYP3A4活性。
使用hepatcult™OGM以多种形式培养类器官,包括在康宁®Matrigel®圆顶中进行常规培养,在旋转瓶中稀释Matrigel®悬浊液中进行放大培养,在96孔板中进行高通量筛选应用,以及在Transwell®插入物上作为类器官衍生的二维单层用于疾病建模应用。类器官也可以冷冻保存以备以后使用,在肝cult™OGM中解冻和培养时,保持高度活活力并保留其进一步分化的能力。
有关肝cult™类器官培养方案的更多信息,请浏览技术手册和教育材料.
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Subtype
Specialized Media
Cell Type
Hepatic Cells
Species
Human
Application
Organoid Culture
Brand
HepatiCult
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
Formulation Category
Serum-Free
Figure 1. HepatiCult™ Organoid Kit (Human) Enables Liver Organoid Initiation, Expansion, and Differentiation
Human liver organoids can be grown from normal human liver tissue-derived hepatic ducts using the HepatiCult™ Organoid Kit (Human). (A) Cultures are established in HepatiCult™ Organoid Initiation Medium (OIM; Human) or HepatiCult™ Organoid Growth Medium (OGM; Human) (see Table 1 below) and subsequently passaged in HepatiCult™ OGM for expansion. (B) After passaging 2-3 times in HepatiCult™ OGM, cultures can be switched to HepatiCult™ Organoid Differentiation Medium (ODM; Human) to differentiate organoids towards more mature hepatic cell types. Refer to the Product Manual (Document #10000008301) for full culturing protocols.
Table 1. Product Recommendations for Liver Organoid Initiation, Expansion, and Differentiation
The recommended configuration of the HepatiCult™ Organoid Kit (Human) may differ based on starting material and experimental goals. When establishing liver organoid cultures from human liver tissue, HepatiCult™ Organoid Initiation Medium (OIM; Human) is recommended for efficient organoid initiation (see Figure 2 below). The expansion of already established organoids (fresh in culture or cryopreserved) is supported by HepatiCult™ Organoid Growth Medium (OGM; Human). These organoids should be maintained for 2-3 passages before further differentiation using HepatiCult™ Organoid Differentiation Medium (ODM). Refer to the Product Manual (Document #10000008301) for full culturing protocols.
Figure 2. HepatiCult™ Organoid Kit (Human) Provides Efficient Organoid Initiation From Human Liver Tissue
(A) Organoid cultures were initiated in HepatiCult™ OIM, and then (B) passaged into HepatiCult™ OGM. For serum-free culture conditions, organoid cultures were both, (C) initiated in and (D) passaged in HepatiCult™ OGM. Culture images shown are from (A, C) day 15 following initiation, and (B, D) on day 8 of the first passage. (E) Quantification of organoid initiation efficiency shows a significantly higher organoid yield in HepatiCult™ OIM per gram of human liver tissue (mean ± SD; n=14).
Figure 3. HepatiCult™ Organoid Initiation Medium (Human) Supports Robust Organoid Establishment Across Multiple Liver Tissue Donor Samples
Organoids initiated from 4 donor tissue samples (A-D) exhibit morphological heterogeneity 15 days after initiation. All initiated cultures were subsequently expanded in HepatiCult™ OGM using a 1:1 passaging ratio (E-H), yielding healthy organoids at the end of the first passage.
Figure 4. Expansion of Organoid Cultures in HepatiCult™ Organoid Growth Medium
Hepatic organoids show efficient growth in HepatiCult™ OGM across multiple donors and passages with potential for indefinite culture. Organoids initiated using the serum-free workflow exhibited comparable growth rates when expanded in HepatiCult™ OGM (open markers).
Figure 5. Proliferating Hepatic Organoids Display Characteristics of Hepatic Progenitors
Human liver organoids grown in HepatiCult™ OGM display characteristics of proliferating hepatic progenitors observed through immunocytochemistry staining of (A) KI67, (B) HNF4A and (C) SOX9. Proliferating hepatic organoids also display characteristics of the hepatic epithelium including expression of (A) EPCAM. (B, C) Nuclei are counterstained with DAPI.
Figure 6. Proliferating Hepatic Organoids Maintain Genetic Expression Across Multiple Passages
Liver organoids maintained in HepatiCult™ OGM express stem cell markers (A) LGR5 and (B) AXIN2, ductal markers (C) SOX9 and (D) KRT19, as well as hepatic marker (E) HNF4a and (F) Albumin (ALB) across multiple passages, with minimal albumin expression observed during culture in HepatiCult™ OGM. Expression levels were measured by qPCR and normalized to TBP and UBC housekeeping genes to quantify relative expression levels. (mean ± SD; n = 2-5 organoid lines), * p < 0.05.
Figure 7. Organoid Differentiation Induces Changes in Organoid Morphology
Organoids exhibit a compact and dense morphology, often comprising thickened epithelia, upon switching cultures to HepatiCult™ Organoid Differentiation Medium (ODM). Shown are images of the same culture well over the course of the differentiation, including (A) day two of culture in HepatiCult™ OGM, (B) day five of culture, immediately after switching organoid cultures from HepatiCult™ OGM to HepatiCult™ ODM, (C) day seven of culture (two days after switching to ODM), (D) day ten of culture (five days after switching to ODM), and (E) day 15 of culture (ten days after switching to ODM). (F) Magnification of the rectangular section highlighted in (E).
Figure 8. Differentiation of Hepatic Organoids in HepatiCult™ ODM Induces Changes in Gene Expression Consistent With Hepatic Maturation
Upon differentiation in HepatiCult™ ODM, liver organoids show changes in gene expression with a decrease in expression of stem cell marker LGR5, as well as of ductal markers CK19, CK7, and SOX9, and an increase in expression of hepatic markers HNF4A, ALB, CYP3A4, and ASGR1. Each replicate represents an individual donor sample analyzed by qPCR at passage 4 on day 8 in HepatiCult™ OGM and day 15 in HepatiCult™ ODM, showing relative gene expression levels normalized to TBP and UBC housekeeping genes.
Figure 9. Differentiated Hepatic Organoids Demonstrate Functionality of Mature Hepatocytes
Upon differentiation in HepatiCult™ ODM, liver organoids were assayed for (A) albumin secretion, (B) CYP3A4 activity, (C) total bile acid production, and (D) urea production. Hepatic functionalities were compared to HepG2 cells and primary human hepatocytes (PHH), which were cultured in supplier-recommended media. Albumin secretion was detected using an ELISA kit (Abcam), total bile acid and urea production were analysed using colorimetric kits (Abcam), and CYP43A4 activity, referring to baseline activity without induction, was determined using the Luciferin-IPA kit (Promega). (mean ± SD; n = 3 organoid lines across 2 experiments, n = 2-3 technical replicates of HepG2 in 1 experiment, and n = 3 cryopreserved PHH donor samples in 1 experiment), * p < 0.05; ** p < 0.01.
Figure 10. HepatiCult™ Organoid Kit Supports the Growth and Differentiation of Porcine Liver Organoids
Porcine liver organoids were established in HepatiCult™ Organoid Initiation Medium, and subsequently expanded for 4 passages in (A) HepatiCult™ Organoid Growth Medium (Human) and differentiated in (B, C) HepatiCult™ Organoid Differentiation Medium (Human). Organoids are stained with basolateral protein marker p120 (green), apical membrane and bile canaliculi marker F-Actin (purple), and nuclear dye Hoechst (blue). (D) Magnification of Panel C indicates bile canaliculi formation as seen through F-Actin staining. Scale bars = 50 μm. Data used with permission from Dr. Amy Engevik (Vanderbilt University Medical Center)
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
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Formulation Category | Serum-Free |
建立和维持小鼠肝祖类器官的细胞培养基
从人PSCs中产生肝细胞样细胞的无血清分化试剂盒
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