切换导航
  • 比较产品
我的购物车

IntestiCult™类器官分化培养基（人源）

用于人肝脏类器官分化的培养基试剂盒
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #100-0383_C

用于人肝脏类器官分化的培养基试剂盒

产品组分包括

  • HepatiCult™ 类器官基础培养基（人源），95 mL
  • HepatiCult™ 分化添加剂（人源），5 mL
main product photo
Interested in trying STEMCELL's organoid products for your liver research? Fill out this form to request information about introductory offers.
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. HepatiCult™ Organoid Growth Medium (Human)
    HepatiCult™ Organoid Growth Medium (Human)

    Culture medium for long-term expansion and maintenance of human liver organoids

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. Dexamethasone
    Dexamethasone

    Glucocorticoid pathway activator; Activates glucocorticoid receptor

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

使用 HepatiCult™ 类器官分化培养基（ODM）（人源）可稳定生成具有肝脏功能性的成熟人源肝脏类器官。该培养基包含支持新鲜或冻存人肝组织来源的肝脏类器官高效分化所需的所有组分。

HepatiCult™ ODM 既可单独购买，也可作为 HepatiCult™ 类器官试剂盒（人源）的一部分使用，适用于对 HepatiCult™ 类器官起始培养基（人源）中生成的类器官和/或在 HepatiCult™ 类器官生长培养基（人源）中扩增的类器官进行分化培养。经 HepatiCult™ ODM 分化的类器官表达肝细胞标志物（ALB、CYP3A4、MRP4），与未分化类器官相比，其白蛋白分泌水平与 CYP3A4 活性显著上调。这些类器官可适配多种培养方案，包括悬浮培养体系和高通量检测。

有关 HepatiCult™ 类器官培养方案的更多信息，请参阅技术手册与教育资料。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

Subtype
Specialized Media
 
Cell Type
Hepatic Cells
 
Species
Human
 
Application
Organoid Culture
 
Brand
HepatiCult
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
 
Formulation Category
Serum-Free
 

查看更多 显示更少

Data Figures

HepatiCult™ Organoid Kit (Human) Enables Liver Organoid Initiation, Expansion, and Differentiation

Figure 1. HepatiCult™ Organoid Kit (Human) Enables Liver Organoid Initiation, Expansion, and Differentiation

Human liver organoids can be grown from normal human liver tissue-derived hepatic ducts using the HepatiCult™ Organoid Kit (Human). (A) Cultures are established in HepatiCult™ Organoid Initiation Medium (OIM; Human) or HepatiCult™ Organoid Growth Medium (OGM; Human) (see Table 1 below) and subsequently passaged in HepatiCult™ OGM for expansion. (B) After passaging 2-3 times in HepatiCult™ OGM, cultures can be switched to HepatiCult™ Organoid Differentiation Medium (ODM; Human) to differentiate organoids towards more mature hepatic cell types. Refer to the Product Manual (Document #10000008301) for full culturing protocols.

Table 1. Product Recommendations for Liver Organoid Initiation, Expansion, and Differentiation

The recommended configuration of the HepatiCult™ Organoid Kit (Human) may differ based on starting material and experimental goals. When establishing liver organoid cultures from human liver tissue, HepatiCult™ Organoid Initiation Medium (OIM; Human) is recommended for efficient organoid initiation (see Figure 2 below). The expansion of already established organoids (fresh in culture or cryopreserved) is supported by HepatiCult™ Organoid Growth Medium (OGM; Human). These organoids should be maintained for 2-3 passages before further differentiation using HepatiCult™ Organoid Differentiation Medium (ODM). Refer to the Product Manual (Document #10000008301) for full culturing protocols.

HepatiCult™ Organoid Kit (Human) Provides Efficient Organoid Initiation From Human Liver Tissue

Figure 2. HepatiCult™ Organoid Kit (Human) Provides Efficient Organoid Initiation From Human Liver Tissue

(A) Organoid cultures were initiated in HepatiCult™ OIM, and then (B) passaged into HepatiCult™ OGM. For serum-free culture conditions, organoid cultures were both, (C) initiated in and (D) passaged in HepatiCult™ OGM. Culture images shown are from (A, C) day 15 following initiation, and (B, D) on day 8 of the first passage. (E) Quantification of organoid initiation efficiency shows a significantly higher organoid yield in HepatiCult™ OIM per gram of human liver tissue (mean ± SD; n=14).

HepatiCult™ Organoid Initiation Medium (Human) Supports Robust Organoid Establishment

Figure 3. HepatiCult™ Organoid Initiation Medium (Human) Supports Robust Organoid Establishment Across Multiple Liver Tissue Donor Samples

Organoids initiated from 4 donor tissue samples (A-D) exhibit morphological heterogeneity 15 days after initiation. All initiated cultures were subsequently expanded in HepatiCult™ OGM using a 1:1 passaging ratio (E-H), yielding healthy organoids at the end of the first passage.

Expansion of Organoid Cultures in HepatiCult™ Organoid Growth Medium

Figure 4. Expansion of Organoid Cultures in HepatiCult™ Organoid Growth Medium

Hepatic organoids show efficient growth in HepatiCult™ OGM across multiple donors and passages with potential for indefinite culture. Organoids initiated using the serum-free workflow exhibited comparable growth rates when expanded in HepatiCult™ OGM (open markers).

Proliferating Hepatic Organoids Display Characteristics of Hepatic Progenitors

Figure 5. Proliferating Hepatic Organoids Display Characteristics of Hepatic Progenitors

Human liver organoids grown in HepatiCult™ OGM display characteristics of proliferating hepatic progenitors observed through immunocytochemistry staining of (A) KI67, (B) HNF4A and (C) SOX9. Proliferating hepatic organoids also display characteristics of the hepatic epithelium including expression of (A) EPCAM. (B, C) Nuclei are counterstained with DAPI.

Proliferating Hepatic Organoids Maintain Genetic Expression Across Multiple Passages

Figure 6. Proliferating Hepatic Organoids Maintain Genetic Expression Across Multiple Passages

Liver organoids maintained in HepatiCult™ OGM express stem cell markers (A) LGR5 and (B) AXIN2, ductal markers (C) SOX9 and (D) KRT19, as well as hepatic marker (E) HNF4a and (F) Albumin (ALB) across multiple passages, with minimal albumin expression observed during culture in HepatiCult™ OGM. Expression levels were measured by qPCR and normalized to TBP and UBC housekeeping genes to quantify relative expression levels. (mean ± SD; n = 2-5 organoid lines), * p < 0.05.

Organoid Differentiation Induces Changes in Organoid Morphology

Figure 7. Organoid Differentiation Induces Changes in Organoid Morphology

Organoids exhibit a compact and dense morphology, often comprising thickened epithelia, upon switching cultures to HepatiCult™ Organoid Differentiation Medium (ODM). Shown are images of the same culture well over the course of the differentiation, including (A) day two of culture in HepatiCult™ OGM, (B) day five of culture, immediately after switching organoid cultures from HepatiCult™ OGM to HepatiCult™ ODM, (C) day seven of culture (two days after switching to ODM), (D) day ten of culture (five days after switching to ODM), and (E) day 15 of culture (ten days after switching to ODM). (F) Magnification of the rectangular section highlighted in (E).

Differentiation of Hepatic Organoids in HepatiCult™ ODM Induces Changes in Gene Expression Consistent With Hepatic Maturation

Figure 8. Differentiation of Hepatic Organoids in HepatiCult™ ODM Induces Changes in Gene Expression Consistent With Hepatic Maturation

Upon differentiation in HepatiCult™ ODM, liver organoids show changes in gene expression with a decrease in expression of stem cell marker LGR5, as well as of ductal markers CK19, CK7, and SOX9, and an increase in expression of hepatic markers HNF4A, ALB, CYP3A4, and ASGR1. Each replicate represents an individual donor sample analyzed by qPCR at passage 4 on day 8 in HepatiCult™ OGM and day 15 in HepatiCult™ ODM, showing relative gene expression levels normalized to TBP and UBC housekeeping genes.

Differentiated Hepatic Organoids Demonstrate Functionality of Mature Hepatocytes

Figure 9. Differentiated Hepatic Organoids Demonstrate Functionality of Mature Hepatocytes

Upon differentiation in HepatiCult™ ODM, liver organoids were assayed for (A) albumin secretion, (B) CYP3A4 activity, (C) total bile acid production, and (D) urea production. Hepatic functionalities were compared to HepG2 cells and primary human hepatocytes (PHH), which were cultured in supplier-recommended media. Albumin secretion was detected using an ELISA kit (Abcam), total bile acid and urea production were analysed using colorimetric kits (Abcam), and CYP43A4 activity, referring to baseline activity without induction, was determined using the Luciferin-IPA kit (Promega). (mean ± SD; n = 3 organoid lines across 2 experiments, n = 2-3 technical replicates of HepG2 in 1 experiment, and n = 3 cryopreserved PHH donor samples in 1 experiment), * p < 0.05; ** p < 0.01.

HepatiCult™ Organoid Kit Supports the Growth and Differentiation of Porcine Liver Organoids

Figure 10. HepatiCult™ Organoid Kit Supports the Growth and Differentiation of Porcine Liver Organoids

Porcine liver organoids were established in HepatiCult™ Organoid Initiation Medium, and subsequently expanded for 4 passages in (A) HepatiCult™ Organoid Growth Medium (Human) and differentiated in (B, C) HepatiCult™ Organoid Differentiation Medium (Human). Organoids are stained with basolateral protein marker p120 (green), apical membrane and bile canaliculi marker F-Actin (purple), and nuclear dye Hoechst (blue). (D) Magnification of Panel C indicates bile canaliculi formation as seen through F-Actin staining. Scale bars = 50 μm. Data used with permission from Dr. Amy Engevik (Vanderbilt University Medical Center)

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
HepatiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0383
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
HepatiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0383
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
HepatiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0383
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
HepatiCult™ Organoid Differentiation Medium (Human)
Catalog #
100-0383
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

Resources and Publications

Educational Materials (19)

Organoids
Brochure
Organoids
Cryopreserving Human Liver Tissue for Organoid Culture
Protocol
Cryopreserving Human Liver Tissue for Organoid Culture
How to Perform Cytotoxicity Screening Using Hepatic Organoids Cultured in HepatiCult™ (Human)
Protocol
How to Perform Cytotoxicity Screening Using Hepatic Organoids Cultured in HepatiCult™ (Human)
Expansion of Hepatic Organoids via Single Cells Using HepatiCult™ Organoid Growth Medium
Protocol
Expansion of Hepatic Organoids via Single Cells Using HepatiCult™ Organoid Growth Medium
Cryopreserving Hepatic Organoids Expanded in HepatiCult™ Organoid Growth Medium (Human)
Protocol
Cryopreserving Hepatic Organoids Expanded in HepatiCult™ Organoid Growth Medium (Human)
Detection of CYP3A4 Activity in Human Hepatic Organoids by LC-MS
Protocol
Detection of CYP3A4 Activity in Human Hepatic Organoids by LC-MS
CRISPR-Cas9 Genome Editing of Human Liver Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Liver Organoids
Establishment of 3D Organoid-Derived Hepatic Monolayers Using HepatiCult™ Organoid Initiation Medium (Human)
Protocol
Establishment of 3D Organoid-Derived Hepatic Monolayers Using HepatiCult™ Organoid Initiation Medi...
Recovery and Expansion of Hepatic Organoids Using HepatiCult™ Organoid Growth Medium
Protocol
Recovery and Expansion of Hepatic Organoids Using HepatiCult™ Organoid Growth Medium
How to Perform Albumin Detection Assays in Human Hepatic Organoids
Protocol
How to Perform Albumin Detection Assays in Human Hepatic Organoids
How to Establish, Expand, and Cryopreserve hPSC-Derived Liver Organoids
Protocol
How to Establish, Expand, and Cryopreserve hPSC-Derived Liver Organoids
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
Expansion of Hepatic Organoids in Dilute Matrigel® Suspension Cultures Using HepatiCult™ Organoid Growth Medium
Protocol
Expansion of Hepatic Organoids in Dilute Matrigel® Suspension Cultures Using HepatiCult™ Organoid...
Predictive In Vitro Models for Toxicity Testing and Liver Research
1:03:03
Webinar
Predictive In Vitro Models for Toxicity Testing and Liver Research
Hepatic Organoids: Improved Tools and In Vitro Models for Drug Development
16:13
Webinar
Hepatic Organoids: Improved Tools and In Vitro Models for Drug Development
Engineering Organoids Using ECM from Decellularized Tissue
36:39
Webinar
Engineering Organoids Using ECM from Decellularized Tissue
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch
Hepatic Organoid Course
On-Demand Training
Hepatic Organoid Course

更多信息

更多信息
Species Human
Formulation Category Serum-Free
Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right touse it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use shouldcontact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。