HemaTox™髓系试剂盒

用于测定药物髓系造血毒性的无血清培养基及补充剂

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产品号 #（选择产品）

产品号 #09704_C

用于测定药物髓系造血毒性的无血清培养基及补充剂

产品组分包括

HemaTox™髓系培养基，100 mL
HemaTox™髓系100X补充剂，1 mL

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总览

该产品为筛选大量新药化合物对人造血干祖细胞（HSPC）增殖和往髓系定向分化的影响而设计。

与使用集落形成单位（CFU）实验相比，基于液体培养的HemaTox™实验更加灵活：您可以在整个培养过程的不同时间点添加测试化合物，测定其对更原始或更成熟的祖细胞的影响。HemaTox™髓系试剂盒促进CD34+ HSPC增殖以及分化为表达髓系标志物（如CD13、CD14和CD15）的细胞。

用户可以自选读出方法来量化细胞对每个待测化合物的反应以及估计化合物的IC50和IC90。每套试剂盒可在5块96孔板中测试多达160个条件（三复孔）。该试剂盒可单独使用或与HemaTox™红系试剂盒（产品号 #09701）或HemaTox™巨核细胞试剂盒（产品号 #09707）联合使用，以平行评估谱系特异性药物造血毒性。

Data Figures

Figure 1. General HemaTox™ Myeloid Kit Procedure

*The cell isolation step may be omitted if pre-enriched CD34+ cells are used.

Figure 2. Flow Cytometry Plot Showing Cells Produced After Culture of CD34+ HSPCs with the HemaTox™ Myeloid Kit

(A) Human CB CD34+ cells were cultured with the HemaTox™ Myeloid Kit using the protocol as written in the Product Information Sheet (PIS). (B) After the appropriate culture period, cells were harvested and stained for cell surface proteins expressed on myeloid (CD13 and CD15) cells.

Figure 3. Reproducibility of HemaTox™ Myeloid Kit Results Between Experiments and Using Different CD34+ Cell Preparations

(A) Dose-response curves were generated from titrations of 5-FU added to human CB CD34+ cells isolated from 3 donors and cultured with the HemaTox™ Myeloid Kit. Three to five separate experiments were performed with cells from each donor. In each assay similar IC50 values were obtained with cells from different donors and in different experiments with cells from the same donor. Shown are values normalized to the percentages (%) of maximum cell growth without drug. Despite differences in absolute cell counts, curves are reproducible when normalized within each experiment. (B) Table showing IC50 values generated for 5-FU in culture with the myeloid kit including standard deviation (SD) and the coefficient of variation (% CV) across experiments.

Figure 4. Correlation Between IC50 Values for Six Drugs Measured Using the CFU-GM Assay and the 96-Well Plate Liquid Culture-Based HemaTox™ Myeloid Kit

Human BM CD34+ cells were cultured in colony-forming unit - granulocyte/macrophage (CFU-GM) assays with MethoCult™ medium and in liquid culture with the HemaTox™ Myeloid Kit. IC50 values generated using each assay were plotted on the X and Y axes and shown to be highly correlated with a coefficient of determination (R2) of 0.91.

Figure 5. Lineage-Specific Differences in Hematotoxicity Identified with HemaTox™ Erythroid, Myeloid and Megakaryocyte Kits

Results show average IC50 values for each drug tested on human BM CD34+ cells using the HemaTox™ Erythroid (grey), Myeloid (gold) and Megakaryocyte (orange) Kits. Most drugs show similar toxicity for each lineage but some, such as Sunitinib, are ~100-fold more toxic for erythroid than for megakaryocyte progenitor differentiation with intermediate toxicity for myeloid progenitor differentiation. Vertical lines indicate standard error of the mean (SEM) (n = 4 - 8).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Product Information Sheet

Product Name

HemaTox™ Myeloid Kit

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