The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Data Figures
Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Rat IgM Depletion Kit
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Rat IgM Depletion Kit
Catalog #
18644
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (8)
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