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SepMate™-15(试管)

用于体外诊断（IVD）应用的密度梯度离心管
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产品号 #（选择产品）

产品号 #85415_C

用于体外诊断（IVD）应用的密度梯度离心管

产品优势

  • 无需在密度梯度介质上仔细分层血液（例如：lymphooprep™等）。
  • 减少总离心机时间到10分钟与制动新鲜样品
  • 通过简单地倒出上清，可以快速和容易地收获分离的单个核细胞
  • 能否与RosetteSep™浓缩鸡尾酒结合，在30分钟内分离特定细胞类型

产品组分包括

  • SepMate™-15 (IVD)， 100支（目录#85415）
    • 分配器盒包含4袋，25支/袋
  • SepMate™-15 (IVD)， 500支（目录#85420）
    • 分配器盒包含4袋，25管/袋（目录#85415）x 5
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Try SepMate™-15 (IVD) tubes for density gradient centrifugation in your IVD applications. Request a Sample
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Lymphoprep™
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

  2. Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
    Dulbecco's Phosphate Buffered Saline with 2% ...

    Cell culture buffer

What Our Scientist Says

Traditional isolation of PBMCs requires careful layering of blood onto density gradient media prior to centrifugation. We developed SepMate™ to simplify this process, so anyone can isolate PBMCs with a simple pour while maintaining consistency across samples.

Peter MorinTechnical Scientist
Peter Morin, Technical Scientist
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

通过将SepMate™纳入您的密度梯度离心步骤，简化外周血单个核细胞（PBMC）分离。

SepMate™管包含一个插入物，在密度梯度介质和血液之间形成屏障，从而消除了仔细分层血液的需要，并允许简单的倒液即可轻松收获单个核细胞。本品可与RosetteSep™分离特异性免疫细胞亚群。

SepMate™在cGMP下生产，并在澳大利亚、加拿大、欧洲和美国注册为体外诊断（IVD）设备。在中国，SepMate™被中国食品药品监督管理局（CFDA）视为非医疗器械，应作为一般实验室设备使用。最终用户负责确定产品是否适合他们的特定应用。

浏览我们的常见问题（FAQs）在SepMate™。

Contains

Polypropylene tube containing an insert
 
Subtype
Centrifugation Tubes
 
Cell Type
B Cells, Dendritic Cells, Monocytes, Mononuclear Cells, NK Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets, T Cells, Regulatory
 
Species
Human
 
Sample Source
Bone Marrow, Whole Blood
 
Selection Method
Negative
 
Application
Cell Isolation, In Vitro Diagnostic
 
Brand
SepMate
 
Area of Interest
Chimerism, HLA, Immunology
 
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Data Figures

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 1. Recovery of mononuclear cells (MNCs) from peripheral blood using SepMate™-50 versus standard density gradient centrifiguation.

Recovery of MNCs from fresh and 48-hour post blood draw enriched by density gradient centrifugation with SepMate™ (purple) or without (grey). There was no significant difference in the recovery of MNCS with and without SepMate™.

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 2. Human CD4+ T Cell Isolation using SepMate™-50 and RosetteSep™ Human CD4+ T Cell Enrichment Cocktail

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
SepMate™-15 (IVD)
Catalog #
85415, 85420
Lot #
All
Language
MULTI

Resources and Publications

Educational Materials (13)

SepMate™ Hassle-Free PBMCs in Just 15 Minutes
Brochure
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
Brochure
Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Protocol
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
2:28
Video
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
1:02
Video
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
How to Speed up Your PBMC Isolations with SepMate™
18:36
Video
How to Speed up Your PBMC Isolations with SepMate™
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populations
Scientific Poster
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populat...
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course

Publications (30)

A highly potent lymphatic system-targeting nanoparticle cyclosporine prevents glomerulonephritis in mouse model of lupus. R. Ganugula et al. Science advances 2020 jun

Abstract

Cyclosporine A (CsA) is a powerful immunosuppressant, but it is an ineffective stand-alone treatment for systemic lupus erythematosus (SLE) due to poor target tissue distribution and renal toxicity. We hypothesized that CD71 (transferrin receptor 1)-directed delivery of CsA to the lymphatic system would improve SLE outcomes in a murine model. We synthesized biodegradable, ligand-conjugated nanoparticles [P2Ns-gambogic acid (GA)] targeting CD71. GA conjugation substantially increased nanoparticle association with CD3+ or CD20+ lymphocytes and with intestinal lymphoid tissues. In orally dosed MRL-lpr mice, P2Ns-GA-encapsulated CsA increased lymphatic drug delivery 4- to 18-fold over the ligand-free formulation and a commercial CsA capsule, respectively. Improved lymphatic bioavailability of CsA was paralleled by normalization of anti-double-stranded DNA immunoglobulin G titer, plasma cytokines, and glomerulonephritis. Thus, this study demonstrates the translational potential of nanoparticles that enhance the targeting of lymphatic tissues, transforming CsA into a potent single therapeutic for SLE.
View publication
A dominant autoinflammatory disease caused by non-cleavable variants of RIPK1 P. Tao et al. Nature 2020

Abstract

Activation of RIPK1 controls TNF-mediated apoptosis, necroptosis and inflammatory pathways1. Cleavage of human and mouse RIPK1 after residues D324 and D325, respectively, by caspase-8 separates the RIPK1 kinase domain from the intermediate and death domains. The D325A mutation in mouse RIPK1 leads to embryonic lethality during mouse development2,3. However, the functional importance of blocking caspase-8-mediated cleavage of RIPK1 on RIPK1 activation in humans is unknown. Here we identify two families with variants in RIPK1 (D324V and D324H) that lead to distinct symptoms of recurrent fevers and lymphadenopathy in an autosomal-dominant manner. Impaired cleavage of RIPK1 D324 variants by caspase-8 sensitized patients' peripheral blood mononuclear cells to RIPK1 activation, apoptosis and necroptosis induced by TNF. The patients showed strong RIPK1-dependent activation of inflammatory signalling pathways and overproduction of inflammatory cytokines and chemokines compared with unaffected controls. Furthermore, we show that expression of the RIPK1 mutants D325V or D325H in mouse embryonic fibroblasts confers not only increased sensitivity to RIPK1 activation-mediated apoptosis and necroptosis, but also induction of pro-inflammatory cytokines such as IL-6 and TNF. By contrast, patient-derived fibroblasts showed reduced expression of RIPK1 and downregulated production of reactive oxygen species, resulting in resistance to necroptosis and ferroptosis. Together, these data suggest that human non-cleavable RIPK1 variants promote activation of RIPK1, and lead to an autoinflammatory disease characterized by hypersensitivity to apoptosis and necroptosis and increased inflammatory response in peripheral blood mononuclear cells, as well as a compensatory mechanism to protect against several pro-death stimuli in fibroblasts.
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Rheumatoid Arthritis Patients, Both Newly Diagnosed and Methotrexate Treated, Show More DNA Methylation Differences in CD4+ Memory Than in CD4+ Na\ive T Cells." K. Guderud et al. Frontiers in immunology 2020

Abstract

Background: Differences in DNA methylation have been reported in B and T lymphocyte populations, including CD4+ T cells, isolated from rheumatoid arthritis (RA) patients when compared to healthy controls. CD4+ T cells are a heterogeneous cell type with subpopulations displaying distinct DNA methylation patterns. In this study, we investigated DNA methylation using reduced representation bisulfite sequencing in two CD4+ T cell populations (CD4+ memory and na{\{i}}ve cells) in three groups: newly diagnosed disease modifying antirheumatic drugs (DMARD) na{\"{i}}ve RA patients (N = 11) methotrexate (MTX) treated RA patients (N = 18) and healthy controls (N = 9) matched for age gender and smoking status. Results: Analyses of these data revealed significantly more differentially methylated positions (DMPs) in CD4+ memory than in CD4+ na{\""{i}}ve T cells (904 vs. 19 DMPs) in RA patients compared to controls. The majority of DMPs (72{\%}) identified in newly diagnosed and DMARD na{\""{i}}ve RA patients with active disease showed increased DNA methylation (39 DMPs) whereas most DMPs (80{\%}) identified in the MTX treated RA patients in remission displayed decreased DNA methylation (694 DMPs). Interestingly we also found that about one third of the 101 known RA risk loci overlapped (±500 kb) with the DMPs. Notably introns of the UBASH3A gene harbor both the lead RA risk SNP and two DMPs in CD4+ memory T cells. Conclusion: Our results suggest that RA associated DNA methylation differences vary between the two T cell subsets but are also influenced by RA characteristics such as disease activity disease duration and/or MTX treatment."""
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更多信息

更多信息
Species Human
Contains Polypropylene tube containing an insert
Sample Source Bone Marrow, Whole Blood
Selection Method Negative
Legal Statement: SepMate™ (IVD) is only available in regions where it is registered as an In Vitro Diagnostic (IVD) device for the isolation of MNCs from whole blood or bone marrow by density gradient centrifugation. SepMate™ is manufactured under a cGMP quality managment system compliant to 21 CFR 820. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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