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EasySep™人Pan-NK细胞分选试剂盒

8分钟内通过免疫磁珠负选分离人Pan-NK细胞
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产品号 #（选择产品）

产品号 #100-1580_C

8分钟内通过免疫磁珠负选分离人Pan-NK细胞

产品优势

  • 快速、易操作、无需分选柱
  • 纯度高达96%且回收率高
  • 分选后的细胞无磁珠标记

产品组分包括

  • EasySep™人Pan-NK细胞分选试剂盒（产品号 #100-1580）
    • EasySep™人Pan-NK细胞分选抗体混合物，1 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103 磁珠，2 x 1 mL
  • RoboSep™人Pan-NK细胞分选试剂盒（产品号 #100-1662）
    • EasySep™人Pan-NK细胞分选抗体混合物，1 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103 磁珠，2 x 1 mL
    • RoboSep™ 过滤吸头（产品号 #20125）x 2
    • RoboSep™ 缓冲液（产品号 #20104）
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总览
实验数据
产品说明书及文档
相关产品

总览

可简便高效地从新鲜或冻存的外周血单个核细胞 (PBMCs) 或白细胞单采术样本中分离出高纯度的人CD56+自然杀伤 (NK) 细胞亚群（包括HLA-DR+和CD36+新兴亚群）。通过EasySep™人类泛NK细胞分选试剂盒的免疫磁珠阴性分选技术实现该分选。EasySep™无柱免疫磁珠分选技术结合单克隆抗体的特异性和无需分选柱的简便性，20多年来被广泛引用于已发表的文献中。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需简单地将目的细胞倾倒或吸取至一个新的试管中,仅需8分钟即可获得高纯度的T细胞，且可立即用于流式细胞术、NK细胞培养、NK细胞扩增、DNA/RNA提取等下游应用，具体取决于研究需求。

了解更多关于EasySep™免疫磁珠分选技术原理，或如何通过RoboSep™实现全自动免疫磁珠细胞分选。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

磁体兼容性
EasySep™ Magnet (Catalog #18000) “The Big Easy” EasySep™ Magnet (Catalog #18001) EasyEights™ EasySep™ Magnet (Catalog #18103) Easy 50 EasySep™ Magnet (Catalog #18002) RoboSep™-S (Catalog #21000)
 
细胞类型
NK 细胞
 
种属
人
 
样本来源
Leukapheresis，PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
癌症，免疫
 

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Data Figures

Flow cytometry dot plot data showing highly purified NK cell (CD45+CD3-CD56+) population post EasySep™ Human Pan-NK Cell Isolation.

Figure 1. Typical EasySep™ Human Pan-NK Cell Isolation Profile

Starting with human PBMCs, the NK cell (CD3-CD56+) content of the isolated fraction is typically 89.4. ± 6.6% (mean ± SD). In the above example, the final purities of the start and isolated fractions are 5.6% and 92.4%, respectively.

Flow cytometry data showing NK cells obtained from donors with a high body mass index (BMI) are expected to have a higher proportion of CD36+ NK cells compared to healthy donors.

Figure 2. EasySep™ Human Pan-NK Cell Isolation Profile from a Donor with a High BMI

NK cells obtained from donors with a high body mass index (BMI) are expected to have a higher proportion of CD36+ NK cells compared to healthy donors. Starting with human PBMCs obtained from a donor with a BMI >30, the CD36+CD56+ NK cell content of the start and isolated fractions were (A) 0.3% and (B) 1.2%, respectively. CD36 is also expressed on non-NK cell subsets, including CD14+ cells, which are gated out to accurately measure NK cell content.

Flow cytometry data showing NK cells obtained from donors with systemic lupus erythematosus (SLE) are expected to have a higher proportion of HLA-DR+ NK cells compared to healthy donors.

Figure 3. EasySep™ Human Pan-NK Cell Isolation Profile from an SLE Patient Sample

NK cells obtained from donors with systemic lupus erythematosus (SLE) are expected to have a higher proportion of HLA-DR+ NK cells compared to healthy donors. Starting with human PBMCs obtained from a patient with SLE, the HLA-DR+CD56+ NK cell content of the start and isolated fractions were (A) 1.2% and (B) 1.6%, respectively. HLA-DR is also expressed on non-NK cell subsets, including CD14+ cells, which are gated out to accurately measure the NK cell content.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Pan-NK Isolation Kit
Catalog #
100-1580
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Pan-NK Cell Isolation Kit
Catalog #
100-1662
Lot #
All
Language
English

更多信息

更多信息
Species Human
Magnet Compatibility EasySep™ Magnet (Catalog #18000) “The Big Easy” EasySep™ Magnet (Catalog #18001) EasyEights™ EasySep™ Magnet (Catalog #18103) Easy 50 EasySep™ Magnet (Catalog #18002) RoboSep™-S (Catalog #21000)
Sample Source Leukapheresis, PBMC
Selection Method Negative
标记抗体
  1. 抗人HLA-DR，克隆L243
    抗人HLA-DR，克隆L243

    抗人、恒河猴、食蟹猴HLA-DR的小鼠单克隆IgG2a抗体

  2. 抗人CD36抗体，克隆FA6-152
    抗人CD36抗体，克隆FA6-152

    小鼠单克隆IgG1抗体，抗人、大鼠CD36

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