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EasySep™人髓系正选试剂盒II

免疫磁珠正选试剂盒
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产品号 #（选择产品）

产品号 #17893_C

免疫磁珠正选试剂盒

产品优势

  • 操作简单、快速
  • 纯度高达99%
  • 无需分离柱

产品组分包括

  • EasySep™人髓系正选试剂盒II（产品号 #17893）
    • EasySep™人髓系正选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
  • RoboSep™ 人髓系阳性分选试剂盒II（含过滤吸头）（产品号 #17893RF）
    • EasySep™人髓系正选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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  1. EasySep™磁铁
    EasySep™磁铁
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

EasySep™ 人髓系正选试剂盒II旨在通过正选从氯化铵裂解处理的全血或骨髓中分离CD33+和CD66b+细胞。目的细胞被识别CD33+和CD66b+的抗体四聚体和磁珠标记。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。CD33和CD66b抗原在髓系祖细胞、单核细胞和粒细胞上表达。

该产品可替代EasySep™人髓系正选试剂盒 (产品号 #18653) 以进行更快地细胞分选。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群，髓系细胞
 
种属
人
 
样本来源
PMNC，Whole Blood
 
筛选方法
Positive
 
品牌
EasySep，RoboSep
 
研究领域
嵌合体，HLA，免疫
 

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Data Figures

Starting with a nucleated cell suspension prepared by lysing whole blood, the myeloid cell content (CD14+CD66b+) of the isolated fraction is typically 96.5 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 59.2% and 94.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Myeloid Positive Selection Kit II
Catalog #
17893RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Myeloid Positive Selection Kit II
Catalog #
17893
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
Sample Source PMNC, Whole Blood
Selection Method Positive
标记抗体
  1. 抗人CD14抗体，克隆MoP9
    抗人CD14抗体，克隆MoP9

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2b抗体

  2. 山羊抗小鼠 IgG (H+L) 抗体，多克隆
    山羊抗小鼠 IgG (H+L) 抗体，多克隆

    抗小鼠IgG（H+L）山羊多克隆抗体

  3. 抗人CD66b抗体，克隆G10F5
    抗人CD66b抗体，克隆G10F5

    小鼠单克隆IgM抗体，抗人、黑猩猩CD66b

  4. 抗人CD14抗体，克隆M5E2
    抗人CD14抗体，克隆M5E2

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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