切换导航
  • 比较产品
我的购物车

EasySep™人中枢和效应记忆CD8+ T细胞分离试剂盒

采用颗粒释放技术的免疫磁细胞分离试剂盒
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #17869_C

采用颗粒释放技术的免疫磁细胞分离试剂盒

产品优势

  • 易于使用和无列
  • 纯度高达93%
  • EasySep™releable RapidSpheres™免洗去除

产品组分包括

  • EasySep™人类中枢和效应记忆CD8+ T细胞分离试剂盒（目录#17869）
    • EasySep™人记忆CD8+ T细胞分离试剂盒，0.5 mL
    • EasySep™Dextran RapidSpheres™，1ml
    • EasySep™Human CD62L阳性选择试剂盒，1ml
    • EasySep™可释放RapidSpheres™，1ml
    • EasyeSep™释放缓冲液，1ml
main product photo
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

该试剂盒从新鲜外周血单个核细胞（PBMCs）或洗过的白细胞分离样品中分离人CD62L+CD45RO+和CD62L-CD45RO+记忆CD8+ T细胞。首先，使用抗体复合物和EasySep™releable RapidSpheres™通过无柱免疫磁阳性选择分离CD62L+细胞。然后，从EasySep™分离的CD62L+细胞中去除结合的磁性颗粒，使用抗体复合物和EasySep™Dextran RapidSpheres™靶向不需要的CD45RA+和非cd8 + T细胞。最终分离的部分含有高度纯化的CD62L+CD45RO+中央记忆CD8+ T细胞，可立即用于下游应用。效应记忆CD8+ T细胞可以从CD62L-部分分离出来。

Magnet Compatibility

• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
Subtype
Cell Isolation Kits
 
Cell Type
T Cells, CD8+
 
Species
Human
 
Sample Source
Leukapheresis, PBMC
 
Selection Method
Positive
 
Application
Cell Isolation
 
Brand
EasySep
 
Area of Interest
Immunology
 
查看更多 显示更少

Data Figures

Starting with fresh PBMCs, the central memory CD8+ T cell content (CD3+CD8+CD45RO+CD62L+) of the isolated fraction is typically 86.8 ± 8.4% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD8+CD45RO+CD62L-) of the isolated fraction is typically 88.7 ± 6.5% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
Sample Source Leukapheresis, PBMC
Selection Method Positive
标记抗体
  1. 抗人CD45RO抗体，克隆UCHL1
    抗人CD45RO抗体，克隆UCHL1

    小鼠抗人、黑猩猩、普通狨猴CD45RO单克隆IgG2a抗体

  2. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠抗人、黑猩猩CD45单克隆IgG1抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。