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EasySep™人嗜碱性粒细胞分选试剂盒

13分钟免疫磁珠负选细胞分选试剂盒
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产品号 #（选择产品）

产品号 #17969_C

13分钟免疫磁珠负选细胞分选试剂盒

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达98%
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™人嗜碱性粒细胞分选试剂盒（产品号 #17969）
    • EasySep™人嗜碱性粒细胞分选抗体混合物l, 1 mL
    • EasySep™ Dextran RapidSpheres™      磁珠, 2 x 1 mL
  • RoboSep™ 人嗜碱性粒细胞分选试剂盒（含过滤吸头）（#17969RF）
    • EasySep™人嗜碱性粒细胞分选抗体混合物l, 1 mL
    • EasySep™ Dextran RapidSpheres™ 磁珠, 2 x 1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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总览
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应用领域
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总览

EasySep™ 人嗜碱性粒细胞分选试剂盒旨在 从富含多形核细胞的组分中负选嗜碱性粒细胞。通过识别非嗜碱性粒细胞的四聚体抗体复合物和EasySep™ Dextran RapidSpheres™磁珠靶向去除非目的细胞。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。将所需目的细胞可 简单地倾倒至新试管中。

该试剂盒适用于使用 HetaSep™（产品号 #     ）新鲜外周血沉降法处理的细胞。

该试剂盒可替代EasySep™人嗜碱性粒细胞富集试剂盒 (产品号 #19069     ) 以实行更快速地细胞分选。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群
 
种属
人
 
样本来源
PMNC，Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Data Figures

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
2:10
Video
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Publications (1)

miR-3934 regulates the apoptosis and secretion of inflammatory cytokines of basophils via targeting RAGE in asthma. L. Dou et al. Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 2022 aug

Abstract

BACKGROUND Several miRNAs are now known to have clear connections to the pathogenesis of asthma. The present study focused on the potential role of miR-3934 during asthma development. METHODS miR-3934 was detected as a down-regulated miRNA in basophils by sequencing analysis. Next, the expression levels of miR-3934 in peripheral blood mononuclear cells of 50 asthma patients and 50 healthy volunteers were examined by RT-qPCR methods. The basophils were then treated with AGEs and transfected with miR-3934 mimics. The apoptosis levels were examined by flow cytometry assay; and the expression levels of cytokines were detected using the ELISA kits. Finally, the Western blot was performed to examined the expression of key molecules in the TGF-$\beta$/Smad signaling pathway. RESULTS miR-3934 was down-regulated in the basophils of asthmatic patients. The expression of the pro-inflammatory cytokines IL-6, IL-8 and IL-33 was enhanced in basophils from asthmatic patients, and this effect was partially reversed by transfection of miR-3934 mimics. Furthermore, receiver operating characteristics analysis showed that miR-3934 levels can be used to distinguish asthma patients from healthy individuals. miR-3934 partially inhibited advanced glycation end products-induced increases in basophil apoptosis by suppressing expression of RAGE. CONCLUSION Our results indicate that miR-3934 acts to mitigate the pathogenesis of asthma by targeting RAGE and suppressing TGF-$\beta$/Smad signaling.
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更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source PMNC, Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD123（IL-3Rα）抗体，克隆 6H6
    抗人CD123（IL-3Rα）抗体，克隆 6H6

    抗人、恒河猴、白眉猴CD123 （IL-3Rα）的小鼠单克隆IgG1抗体

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