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EasySep™直接人嗜碱性粒细胞分离试剂盒

全血试剂盒免疫磁阴性选择
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产品号 #（选择产品）

产品号 #19667_C

全血试剂盒免疫磁阴性选择

产品优势

  • 有效地消耗红细胞，不需要密度梯度离心，沉淀，或裂解
  • 分离细胞纯度高达98%
  • 快速，易于使用和无列
  • 分离的细胞不受影响

产品组分包括

  • EasySep™直接人嗜碱性粒细胞分离试剂盒（目录#19667）
    • EasySep™直接人嗜碱性粒细胞分离试剂盒，2 × 2.5 mL
    • EasySep™Direct RapidSpheres™50300,4 x 2.5 mL
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

EasySep™直接人嗜碱性粒细胞分离试剂盒通过免疫磁阴性选择直接从人全血中分离功能性、高纯度的嗜碱性粒细胞。不需要裂解、密度梯度离心或其他处理步骤，分离的细胞可立即用于流式细胞术、功能测定、培养和其他下游应用。

Magnet Compatibility

• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
Subtype
Cell Isolation Kits
 
Cell Type
Granulocytes and Subsets
 
Species
Human
 
Sample Source
Whole Blood
 
Selection Method
Negative
 
Application
Cell Isolation
 
Brand
EasySep
 
Area of Interest
Immunology
 
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Data Figures

Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Direct Human Basophil Isolation Kit
Catalog #
19667
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct Human Basophil Isolation Kit
Catalog #
19667
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct Human Basophil Isolation Kit
Catalog #
19667
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Human
Magnet Compatibility • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
Sample Source Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠抗人、黑猩猩CD45单克隆IgG1抗体

  2. 抗人CD123 （IL-3Rα）抗体，克隆6H6
    抗人CD123 （IL-3Rα）抗体，克隆6H6

    小鼠抗人、恒河猴、黑白眉猴CD123 （IL-3Rα）单克隆IgG1抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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