细胞活力检测试剂盒,绿色/红色荧光

用两种荧光染料检测和区分有活力和无活力的细胞

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产品号 #100-1545_C

用两种荧光染料检测和区分有活力和无活力的细胞

总览

细胞活力检测试剂盒,绿色/红色荧光通过使用两种荧光染料(钙黄绿素 AM 和碘化丙啶),可同时检测和区分有活力和无活力的细胞,从而提供了一种方便、可靠的细胞活力检测方法。作为一种荧光染料,钙黄绿素 AM 最初是无荧光的。钙黄绿素 AM 被动进入细胞后,仅存在于活细胞的细胞内酯酶会将其水解为亮绿色荧光分子钙黄绿素(Bratosin et al.)。绿色荧光的强度与酯酶活性成正比,因此可以与存活细胞的数量相关联。碘化丙啶是第二种荧光染料,但与钙黄绿素 AM 不同,碘化丙啶只能穿过受损的死细胞膜。一旦进入死细胞,碘化丙啶就会与 DNA 结合后发出红色荧光。本试剂盒中的染料非常适合与荧光显微镜或荧光酶标仪搭配使用,能够在 FITC(钙黄绿素)和 TRITC(碘化丙啶)通道中进行检测。

使用细胞活力检测试剂盒,绿色/红色荧光可检测和量化贴壁或悬浮培养中的细胞增殖,或将其作为体外细胞毒性检测的测定。

应用
鉴定
 

Data Figures

Fluorescent Microscope Image of Live and Dead Cells in Fluorescent Green and Red

Figure 1. Live or Dead Cell Viability Assay Kit Selectively Labels Live and Dead Cells in Fluorescent Green and Red

(A) Live HEK293 cells (top row) and dead HEK293 cells (bottom row) were stained separately using Cell Viability Assay Kit and then imaged using a fluorescence microscope. Live cells emit fluorescent green, while dead cells with compromised cell membranes emit fluorescent red. (B) Live HEK293 cells were treated with staurosporine, a cytotoxic agent, for 24 hours. Cells were then stained with Cell Viability Assay Kit and imaged using a fluorescence microscope. The merged image shows a mixed population of live and dead cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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100-1545
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Safety Data Sheet 1
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100-1545
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Safety Data Sheet 2
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Safety Data Sheet 3
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Safety Data Sheet 4
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English
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